Global warming, heat waves, and seasonal drought pose serious threats to crops, such as grapevine, that are valued for their secondary metabolites, which are of primary importance for the wine industry. Discriminating the effects of distinct environmental factors in the open field is challenging. In the present study, in vitro cultured berries of Sauvignon Blanc were exposed to individual and combined stress factors to investigate the effects on the biosynthesis of the thiol precursors. Our results confirm the complexity and extreme reactivity of the accumulation process in grapes. However, they also indicate that heat stress has a positive effect on the production of the Cys-3SH precursor. Moreover, we identified several candidate genes, such as VvGSTs and VvGGT that are potentially involved in biosynthesis and consistently modulated. Nonetheless, we were unable to conclusively determine the effects of stresses on the biosynthesis of other precursors nor could we formulate hypotheses regarding their regulation.

中文翻译：

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利用体外培养的浆果揭示热和 ABA 诱导的应激对长相思硫醇前体生物合成的影响


全球变暖、热浪和季节性干旱对葡萄等作物构成严重威胁，这些作物因其次生代谢物而受到重视，而次生代谢物对葡萄酒行业至关重要。区分开放环境中不同环境因素的影响具有挑战性。在本研究中，将体外培养的长相思浆果暴露于单独和组合的应激因素中，以研究对硫醇前体生物合成的影响。我们的结果证实了葡萄中积累过程的复杂性和极端反应性。然而，他们也表明热应激对 Cys-3SH 前体的产生具有积极影响。此外，我们还确定了几个候选基因，例如VvGST和VvGGT ，它们可能参与生物合成并受到持续调节。尽管如此，我们无法最终确定压力对其他前体生物合成的影响，也无法提出有关其调节的假设。