Wheat species with various ploidy levels may be different regarding their immunoreactive potential in celiac disease (CD), but a comprehensive comparison of peptide sequences with known epitopes is missing. Thus, we used an untargeted liquid chromatography tandem mass spectrometry method to analyze the content of peptides with CD-active epitope in the five wheat species common wheat, spelt, durum wheat, emmer, and einkorn. In total, 494 peptides with CD-active epitope were identified. Considering the average of the eight cultivars of each species, spelt contained the highest number of different peptides with CD-active epitope (193 ± 12, mean ± SD). Einkorn showed the smallest variability of peptides (63 ± 4) but higher amounts of certain peptides compared to the other species. The wheat species differ in the presence and distribution of CD-active epitopes; hence, the entirety of peptides with CD-active epitope is crucial for the assessment of their immunoreactive potential.

中文翻译：

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普通小麦、斯佩耳特小麦、硬粒小麦、二粒小麦和单粒小麦中乳糜泻活性表位的比较无标记蛋白质组学研究


具有不同倍性水平的小麦品种在乳糜泻（CD）中的免疫反应潜力可能有所不同，但缺乏肽序列与已知表位的全面比较。为此，我们采用非靶向液相色谱串联质谱方法对普通小麦、斯佩耳特小麦、硬粒小麦、二粒小麦和单粒小麦这5种小麦中CD活性表位肽的含量进行了分析。总共鉴定出 494 种具有 CD 活性表位的肽。考虑到每个物种的八个品种的平均值，斯佩尔特含有最多数量的具有CD活性表位的不同肽（193±12，平均值±SD）。与其他物种相比，单粒小麦的肽变异性最小 (63 ± 4)，但某些肽的含量更高。不同小麦品种的 CD 活性表位的存在和分布有所不同；因此，具有 CD 活性表位的整个肽对于评估其免疫反应潜力至关重要。