BACKGROUNDN6‐Methyladenosine (m6A) is a common messenger RNA (mRNA) modification that affects various physiological processes in stress responses. However, the role of m6A modifications in plants responses to herbivore stress remains unclear.RESULTSHere, we found that an infestation of brown planthopper (Nilaparvata lugens) female adults enhanced the resistance of rice to N. lugens. The m6A methylome analysis of N. lugens‐infested and uninfested rice samples was performed to explore the interaction between rice and N. lugens. The m6A methylation mainly occurred in genes that were actively expressed in rice following N. lugens infestation, while an analysis of the whole‐genomic mRNA distribution of m6A showed that N. lugens infestation caused an overall decrease in the number of m6A methylation sites across the chromosomes. The m6A methylation of genes involved in the m6A modification machinery and several defense‐related phytohormones (jasmonic acid and salicylic acid) pathways was increased in N. lugens‐infested rice compared to that in uninfested rice. In contrast, m6A modification levels of growth‐related phytohormone (auxin and gibberellin) biosynthesis‐related genes were significantly attenuated during N. lugens infestation, accompanied by the down‐regulated expression of these transcripts, indicating that rice growth was restricted during N. lugens attack to rapidly optimize resource allocation for plant defense. Integrative analysis of the differential patterns of m6A methylation and the corresponding transcripts showed a positive correlation between m6A methylation and transcriptional regulation.CONCLUSIONThe m6A modification is an important strategy for regulating the expression of genes involved in rice defense and growth during rice–N. lugens interactions. These findings provide new ideas for formulating strategies to control herbivorous pests. © 2024 Society of Chemical Industry.

中文翻译：

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全转录组 N6-甲基腺苷分析揭示了水稻对褐飞虱（Nilaparvata lugens）侵染的反应中的生长与防御权衡


背景N6-甲基腺苷（m6A）是一种常见的信使RNA（mRNA）修饰，影响应激反应中的各种生理过程。然而，m6A 修饰在植物对食草动物胁迫反应中的作用仍不清楚。 结果在这里，我们发现褐飞虱 (Nilaparvata lugens) 雌性成虫的侵染增强了水稻对褐飞虱的抗性。对褐飞虱侵染和未侵染的水稻样品进行 m6A 甲基化分析，以探索水稻和褐飞虱之间的相互作用。 m6A甲基化主要发生在水稻褐飞虱侵染后活跃表达的基因中，而m6A全基因组mRNA分布分析表明，褐飞虱侵染导致水稻中m6A甲基化位点数量整体减少。染色体。与未侵染的水稻相比，受褐飞虱侵染的水稻中涉及 m6A 修饰机制和几种防御相关植物激素（茉莉酸和水杨酸）途径的基因的 m6A 甲基化有所增加。相比之下，生长相关植物激素（生长素和赤霉素）生物合成相关基因的 m6A 修饰水平在褐飞虱侵染期间显着减弱，并伴随着这些转录本的表达下调，表明水稻生长在褐飞虱侵染期间受到限制。攻击以快速优化植物防御的资源分配。 m6A甲基化差异模式和相应转录本的综合分析表明m6A甲基化与转录调控之间呈正相关。结论m6A修饰是调节水稻N期防御和生长相关基因表达的重要策略。鲁根斯相互作用。 这些发现为制定控制草食性害虫的策略提供了新思路。 © 2024 化学工业协会。