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Development of a model for the ethanol concentration limit as a function of temperature and initial substrate concentration using the yeast Saccharomyces cerevisiae
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2024-06-22 , DOI: 10.1002/bit.28771
Ruan Dionízio SILVA 1 , Flávio Luiz Honorato da Silva 1 , Sharline Florentino de Melo Santos 1 , Alberto Brandão Torres Neto 1 , Julice Dutra Lopes 1 , Josilene de Assis Cavalcante 1
Affiliation  

In this study, a model was developed to simulate the effect of temperature (T$T$) and initial substrate concentration (S0${S}_{0}$) on the ethanol concentration limit (Pmax${P}_{\max }$) using the yeast Saccharomyces cerevisiae. To achieve this, regressions were performed using data provided by other authors for Pmax${P}_{\max }$ to establish a model dependent on T$T$ and S0${S}_{0}$ capable of predicting results with statistical significance. After constructing the model, a response surface was generated to determine the conditions where Pmax${P}_{\max }$ reaches higher values: temperatures between 28°C and 32°C and an initial substrate concentration around 200 g/L. Thus, the proposed model is consistent with the observations that increasing temperatures decrease the ethanol concentration obtained, and substrate concentrations above 200 g/L lead to a reduction in ethanol concentration even at low temperatures such as 28°C.

中文翻译:


使用酿酒酵母开发乙醇浓度极限随温度和初始底物浓度变化的模型



在这项研究中,开发了一个模型来模拟温度的影响( 时间$T$ )和初始底物浓度( S 0 ${S}_{0}$ )对乙醇浓度限值( 最大限度${P}_{\最大}$ )使用酿酒酵母。 为了实现这一目标,使用其他作者提供的数据进行回归最大限度${P}_{\最大}$建立一个模型依赖于时间$T$ S 0 ${S}_{0}$能够预测具有统计显着性的结果。 构建模型后,生成响应面以确定其中的条件最大限度${P}_{\最大}$达到更高的值:温度在 28°C 至 32°C 之间,初始底物浓度约为 200 g/L。因此,所提出的模型与观察结果一致,即升高温度会降低获得的乙醇浓度,并且即使在 28°C 等低温下,底物浓度高于 200 g/L 也会导致乙醇浓度降低。
更新日期:2024-06-22
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