The tobacco aphid () poses a significant threat to global Australian tobacco () output by inflicting damage through leaf twisting and feeding during its early growth stages. To explore potential herbivore resistance in , this study investigates the effects of the elicitor protein MoHrip2, derived from , on the immature growth, survival, and lifespan of . Compared to the control group, MoHrip2 treatments at three concentrations (85.24, 51.14, 25.57 μg/mL) reduced population after 7 days, 14 days, and 21 days. All concentrations significantly reduced aphid colonization rate, except for the lowest elicitor concertation (25.57 μg/mL at 7D). Likewise compared to the control group, the highest concentration of MoHrip2 i.e. 85.24 μg/mL, resulted in a decrease in the growth rates of (second and third-generation). colonized control plants quicker than MoHrip2-treated plants in a host selection test. MoHrip2 (85.24, 51.14, 25.57 μg/mL) concentrations extended nymphal development. The trichomes and wax on MoHrip2-treated leaves made environment hostile. reproduction and colonization were reduced by MoHrip2's leaf surface changes. Electrical penetration graphs (EPGs) against assessed adult reproductive efficiency and pest-pathogen interaction. MoHrip2-treated seedlings exhibited increased amounts of expression of genes involved in jasmonic acid (JA), salicylic acid (SA) pathways indicating the activation of systemic defense against . This study reveals a direct correlation between temperature variations and climatic shifts in plants, highlighting the potential of MoHrip2 in revolutionizing agroecosystems through enhanced pest management and biocontrol strategies. In conclusion, the present study sheds new light on the potential of MoHrip2, derived from , as an effective biocontrol technique against , infesting . These findings provide valuable insights into developing innovative and sustainable solutions for pest management in agricultural practices, considering the evidence presented against , it can be concluded that implementing integrated pest management and utilizing MoHrip2 for bio control in the agroecosystem is an appropriate approach.

中文翻译：

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揭开蛋白质激发子 MoHrip2 的面纱：从 Magnaporthe oryzae 中提取的一种有效生物防治剂可保护澳大利亚烟草 (Nicotiana Benthamiana) 免受烟草蚜虫 (Myzus nicotianae) 侵害


烟蚜 () 在其早期生长阶段通过扭曲叶子和取食造成损害，对全球澳大利亚烟草 () 产量构成重大威胁。为了探索 中潜在的食草动物抗性，本研究调查了源自 的激发子蛋白 MoHrip2 对 中未成熟生长、存活和寿命的影响。与对照组相比，三种浓度（85.24、51.14、25.57 μg/mL）的 MoHrip2 处理在 7 天、14 天和 21 天后种群数量减少。除了最低的激发子协同作用（7D 时为 25.57 μg/mL）外，所有浓度均显着降低了蚜虫定植率。同样，与对照组相比，MoHrip2 的最高浓度（85.24 μg/mL）导致（第二代和第三代）的生长速率下降。在宿主选择测试中，对照植物的定植速度比 MoHrip2 处理的植物更快。 MoHrip2（85.24、51.14、25.57 µg/mL）浓度可延长若虫发育。经过 MoHrip2 处理的叶子上的毛状体和蜡使环境变得恶劣。 MoHrip2 叶表面的变化减少了繁殖和定植。根据评估的成虫繁殖效率和害虫-病原体相互作用的电穿透图（EPG）。 MoHrip2处理的幼苗表现出与茉莉酸（JA）、水杨酸（SA）途径相关的基因表达量增加，表明针对 的系统防御被激活。这项研究揭示了植物中温度变化和气候变化之间的直接相关性，突显了 MoHrip2 通过加强害虫管理和生物防治策略彻底改变农业生态系统的潜力。 总之，本研究为 MoHrip2 的潜力提供了新的线索，MoHrip2 源自作为一种有效的生物防治技术来对抗感染。这些发现为开发农业实践中害虫管理的创新和可持续解决方案提供了宝贵的见解，考虑到提出的证据，可以得出结论，在农业生态系统中实施害虫综合管理和利用 MoHrip2 进行生物控制是一种适当的方法。