MAP30, a ribosome-inactivating protein mainly isolated from the fruits and seeds of bitter gourd (), has many pharmacological properties, such as anti-HIV and anti-tumor activities. Using qPCR analysis, we found that transcription was higher in light, but drastically reduced in darkness. However, the molecular mechanism by which is regulated remains unclear. Using yeast one-hybrid (Y1H) library screening, we found that McHY5, a homolog of AtHY5, bound to the promoter. In addition, McHY5 and its homolog McHY5-X1 are identified as positive regulators binding to the promoter by using Y1H, Electrophoretic mobility shift assay (EMSA) and Dual-Luciferase Reporter (DLR) assay. Due to is not present in we transformed to Col and obtained transgenic line 18. Compared with transcription was largely reduced in whether under light or dark conditions. Moreover, approximately 70 % of the tested bitter gourd germplasms showed consistent expression patterns of / and under light conditions. In contrast to McHY5, McPIF1-X1 and McPIF3 acted as inactivators to inhibit transcription. In this study, we found that transcription was activated by McHY5 and McHY5-X1 in light, while McPIF1-X1 and McPIF3 inhibited transcription mainly in darkness. Conclusively, we established the antagonistic module of McHY5/McHY5-X1 and McPIF1-X1/McPIF3 in transcription regulation. This work expands our understanding of the regulatory mechanism of and provides a valuable conceptual guidance for cultivating high pharmacological activity bitter gourd varieties with high MAP30 protein levels.

中文翻译：

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光通过McHY5/McHY5-X1和McPIF1-X1/McPIF3对MAP30转录的拮抗调节促进苦瓜（Momordica charantia）的药理活性


MAP30 是一种核糖体失活蛋白，主要从苦瓜的果实和种子中分离出来，具有许多药理特性，例如抗 HIV 和抗肿瘤活性。通过 qPCR 分析，我们发现在光照下转录较高，但在黑暗中转录急剧减少。然而，其调节的分子机制仍不清楚。通过酵母单杂交 (Y1H) 文库筛选，我们发现 AtHY5 的同源物 McHY5 与启动子结合。此外，通过使用 Y1H、电泳迁移率变动分析 (EMSA) 和双荧光素酶报告基因 (DLR) 分析，McHY5 及其同源物 McHY5-X1 被鉴定为与启动子结合的正调节因子。由于不存在，我们转化Col并获得转基因品系18。与此相比，无论在光照还是黑暗条件下，转录都大大减少。此外，大约70%的测试苦瓜种质在光照条件下表现出一致的l 和 表达模式。与 McHY5 相比，McPIF1-X1 和 McPIF3 作为失活剂抑制转录。在这项研究中，我们发现McHY5和McHY5-X1在光下转录被激活，而McPIF1-X1和McPIF3主要在黑暗下抑制转录。最终，我们建立了McHY5/McHY5-X1和McPIF1-X1/McPIF3在转录调控中的拮抗模块。这项工作拓展了我们对苦瓜调控机制的理解，并为培育高药理活性、高MAP30蛋白水平的苦瓜品种提供了有价值的概念指导。