Antibiotics are a new type of environmental pollutants. Due to its wide application in many fields, antibiotic residues are ubiquitous in the wastewater environments. Given their potential threat on water ecosystem functioning and public health, the detection of antibiotic residues in wastewater environments has become very necessary. Based on the complexation of Al with flumequine (FLU), ciprofloxacin (CIP) and doxycycline hydrochloride (DOX), their molecular conjugated area were increased and fluorescence intensity were enhanced, combined with synchronous fluorescence spectrometry (SFS) had good selectivity and high sensitivity, a novel method of Al sensitized synchronous fluorescence spectrometry for the determination of FLU, CIP and DOX residues in wastewater was established. When the wavelength difference (Δλ) was selected 115.0 nm, synchronous fluorescence spectra of the three antibiotics could be well separated and the interference of wastewater matrix were eliminated primely. The new SFS made good use of spectral separation instead of conventional chemical separation, and the actual wastewater sample could be directly determined after simple filtration. The experiment results showed that the concentrations of FLU, CIP and DOX in the range of 0.5000–800.0 ng·mL, 0.5000–640.0 ng·mL and 10.00–3500 ng·mL had a good linear relationship with fluorescence intensity. The detection limits of three antibiotics were 0.02054 ng·mL, 0.03956 ng·mL and 0.8524 ng·mL, respectively. Recovery rates of three antibiotics in wastewater samples were 90.72%-98.23%, 88.68%-95.08% and 85.94%-96.70%. The new SFS established in this experiment had the advantages of simple, rapid, sensitive, accurate and good selectivity. Simultaneous and rapid detection of FLU, CIP and DOX residues in wastewater was successfully realized. It had good application prospects in real-time water quality monitoring.

中文翻译：

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新型同步荧光光谱法结合Al3+敏化同时快速测定废水中痕量氟甲喹、环丙沙星和盐酸多西环素残留


抗生素是一种新型环境污染物。由于其在许多领域的广泛应用，抗生素残留在废水环境中普遍存在。鉴于它们对水生态系统功能和公众健康的潜在威胁，废水环境中抗生素残留的检测变得非常有必要。基于Al与氟甲喹（FLU）、环丙沙星（CIP）和盐酸多西环素（DOX）的络合，增加了它们的分子共轭面积，增强了荧光强度，结合同步荧光光谱（SFS）具有良好的选择性和高灵敏度，建立了铝敏化同步荧光光谱法测定废水中FLU、CIP和DOX残留的新方法。当波长差（Δλ）选择115.0 nm时，3种抗生素的同步荧光光谱能够得到很好的分离，并且较好地消除了废水基质的干扰。新的SFS充分利用光谱分离代替传统的化学分离，实际废水样品经过简单过滤后即可直接测定。实验结果表明，FLU、CIP和DOX的浓度在0.5000~800.0 ng·mL、0.5000~640.0 ng·mL和10.00~3500 ng·mL范围内与荧光强度具有良好的线性关系。 3种抗生素的检出限分别为0.02054 ng·mL、0.03956 ng·mL和0.8524 ng·mL。废水样品中3种抗生素的回收率分别为90.72%-98.23%、88.68%-95.08%和85.94%-96.70%。本实验建立的新型SFS具有简单、快速、灵敏、准确、选择性好等优点。 成功实现废水中FLU、CIP、DOX残留的同时快速检测。在实时水质监测中具有良好的应用前景。