Kühn is a pathogen causing rice sheath blight (ShB). Ammonium transporter 1 (AMT1) promotes resistance of rice to ShB by activating ethylene signaling. However, how AMT1 activates ethylene signaling remains unclear. In this study, the indeterminate domain 10 (IDD10)-NAC079 interaction model was used to investigate whether ethylene signaling is modulated downstream of ammonium signaling and modulates ammonium-mediated ShB resistance. RT-qPCR assay was used to identify the relative expression levels of nitrogen and ethylene related genes. Yeast two-hybrid assays, Bimolecular fluorescence complementation (BiFC) and Co-immunoprecipitation (Co-IP) assay were conducted to verify the IDD10-NAC079-calcineurin B-like interacting protein kinase 31 (CIPK31) transcriptional complex. Yeast one-hybrid assay, Chromatin immunoprecipitation (ChIP) assay, and Electrophoretic mobility shift assay (EMSA) were used to verify whether was activated by IDD10 and NAC079. Ethylene quantification assay was used to verify ethylene content in transgenic plants. Genetic analysis is used to detect the response of IDD10, NAC079 and CIPK31 to ShB infestation. IDD10-NAC079 forms a transcription complex that activates to inhibit the ethylene signaling pathway to negatively regulating ShB resistance. CIPK31 interacts and phosphorylates NAC079 to enhance its transcriptional activation activity. In addition, AMT1-mediated ammonium absorption and subsequent N assimilation inhibit the expression of and to activate the ethylene signaling pathway, which positively regulates ShB resistance. The study identified the link between ammonium and ethylene signaling and improved the understanding of the rice resistance mechanism.

中文翻译：

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IDD10-NAC079转录因子复合物通过抑制水稻乙烯信号传导调节纹枯病抗性


Kühn 是一种引起水稻纹枯病 (ShB) 的病原体。铵转运蛋白 1 (AMT1) 通过激活乙烯信号传导促进水稻对 ShB 的抗性。然而，AMT1 如何激活乙烯信号传导仍不清楚。在本研究中，使用不确定结构域 10 (IDD10)-NAC079 相互作用模型来研究乙烯信号传导是否在铵信号传导下游受到调节并调节铵介导的 ShB 抗性。 RT-qPCR 测定用于鉴定氮和乙烯相关基因的相对表达水平。进行酵母双杂交测定、双分子荧光互补 (BiFC) 和免疫共沉淀 (Co-IP) 测定来验证 IDD10-NAC079-钙调磷酸酶 B 样相互作用蛋白激酶 31 (CIPK31) 转录复合物。使用酵母单杂交实验、染色质免疫沉淀（ChIP）实验和电泳迁移率变动实验（EMSA）来验证是否被IDD10和NAC079激活。乙烯定量测定用于验证转基因植物中的乙烯含量。遗传分析用于检测 IDD10、NAC079 和 CIPK31 对 ShB 感染的反应。 IDD10-NAC079 形成转录复合物，激活并抑制乙烯信号通路，从而负向调节 ShB 抗性。 CIPK31 与 NAC079 相互作用并磷酸化，以增强其转录激活活性。此外，AMT1介导的铵吸收和随后的氮同化抑制乙烯信号通路的表达并激活乙烯信号通路，从而正向调节ShB抗性。该研究确定了铵和乙烯信号之间的联系，并提高了对水稻抗性机制的理解。