Point-of-care (POC) diagnostics have emerged as a crucial technology for emerging pathogen detections to enable rapid and on-site detection of infectious diseases. However, current POC devices often suffer from limited sensitivity with poor reliability to provide quantitative readouts. In this paper, we present a self-powered digital loop-mediated isothermal amplification (dLAMP) microfluidic chip (SP-dChip) for the rapid and quantitative detection of nucleic acids. The SP-dChip utilizes a vacuum lung design to passively digitize samples into individual nanoliter wells for high-throughput analysis. The superior digitization scheme is further combined with reverse transcription loop-mediated isothermal amplification (RT-LAMP) to demonstrate dLAMP detection of Zika virus (ZIKV). Firstly, the LAMP assay is loaded into the chip and passively digitized into individual wells. Mineral oil is then pipetted through the chip to differentiate each well as an individual reactor. The chip did not require any external pumping or power input for rapid and reliable results to detect ZIKA RNA as low as 100 copies per μL within one hour. As such, this SP-dChip offers a new class of solutions for truly affordable, portable, and quantitative POC detections for emerging viruses.

中文翻译：

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开发用于检测新出现病毒的自供电数字 LAMP 微流控芯片 (SP-dChip)


即时护理 (POC) 诊断已成为新兴病原体检测的一项关键技术，可实现传染病的快速现场检测。然而，当前的 POC 设备通常灵敏度有限，并且提供定量读数的可靠性较差。在本文中，我们提出了一种自供电数字环介导等温扩增（dLAMP）微流控芯片（SP-dChip），用于快速定量核酸检测。 SP-dChip 采用真空肺设计，将样品被动数字化到单独的纳升孔中，以进行高通量分析。卓越的数字化方案进一步与逆转录环介导的等温扩增 (RT-LAMP) 相结合，以证明 dLAMP 对寨卡病毒 (ZIKV) 的检测。首先，将 LAMP 检测加载到芯片中并被动数字化到各个孔中。然后将矿物油移入芯片，以将每口井区分为单独的反应器。该芯片不需要任何外部泵或电源输入，即可在一小时内快速可靠地检测低至 100 个拷贝/μL 的 ZIKA RNA。因此，这款 SP-dChip 提供了一种新型解决方案，可以真正经济实惠、便携式、定量地对新兴病毒进行 POC 检测。