Excessive hydrogen peroxide (H₂O₂) generated during retinal cell metabolic activity could lead to oxidative degeneration of retinal pigment epithelium (RPE) tissue, a specific pathological process implicated in various retinal diseases resulting in blindness, which can be mitigated by taking dietary antioxidants to prevent inflammation and impaired cellular dysfunction. This study tested the hypothesis that damages induced by oxidative stresses can be mitigated by lutein in a H₂O₂-challenged model, which was based on an ARPE-19 cell monolayer cultured on three-dimensional (3D)-printed fibrous scaffolds. Pretreating these models with lutein (0.5 μM) for 24 h can significantly lower the oxidative stress and maintain phagocytosis and barrier function. Moreover, lutein can modulate the NLRP3 inflammasome, leading to a ∼40% decrease in the pro-inflammatory cytokine (IL-1β and IL-18) levels. Collectively, this study suggests that the 3D RPE model is an effective tool to examine the capability of lutein to modulate cellular functionalities and regulate NLRP3 inflammation.

中文翻译：

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叶黄素在 H2O2 挑战的三维视网膜色素上皮模型中调节细胞功能并调节 NLRP3 炎症小体


视网膜细胞代谢活动过程中产生的过量过氧化氢（H ₂ O ₂ ）可能导致视网膜色素上皮（RPE）组织氧化变性，这是一种与多种视网膜疾病相关的特定病理过程，导致失明，可以通过服用膳食抗氧化剂来缓解预防炎症和受损的细胞功能障碍。本研究测试了这样的假设：叶黄素可以在 H ₂ O ₂挑战模型中减轻氧化应激引起的损伤，该模型基于在三维 (3D) 打印纤维支架上培养的 ARPE-19 细胞单层。用叶黄素 (0.5 μM) 预处理这些模型 24 小时可以显着降低氧化应激并维持吞噬作用和屏障功能。此外，叶黄素可以调节 NLRP3 炎症小体，导致促炎细胞因子（IL-1β 和 IL-18）水平降低约 40%。总的来说，这项研究表明 3D RPE 模型是检查叶黄素调节细胞功能和调节 NLRP3 炎症能力的有效工具。