A Multifunctional Tissue-Engineering Hydrogel Aimed to Regulate Bacterial Ferroptosis-Like Death and Overcoming Infection Toward Bone Remodeling,Advanced Science

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A Multifunctional Tissue-Engineering Hydrogel Aimed to Regulate Bacterial Ferroptosis-Like Death and Overcoming Infection Toward Bone Remodeling
Advanced Science ( IF 14.3 ) Pub Date : 2024-06-19 , DOI: 10.1002/advs.202309820
Renjie Lu _{1,

2} , Zhiyuan Luo ₁ , Yuanyuan Zhang ₂ , Jiahao Chen _{1,

2} , Yang Zhang _{2,

3} , Chi Zhang ₁

Affiliation

Infection is the most common complication after orthopedic surgery and can result in prolonged ailments such as chronic wounds, enlarged bone defects, and osteomyelitis. Iron, which is essential for bacterial metabolism and immune cell functions, is extremely important. Bacteria harness iron from nearby cells to promote biofilm formation, ensuring their survival. Iron deficiency within the infection microenvironment (IME) consequently hampers macrophage function, enabling further dissemination of the infection and hindering macrophage polarization to the M2 phenotype. Therefore, a novel approach is proposed to regulate macrophage polarization, aiming to restore the inflammatory immune environment. A composite hydrogel derived from natural polymers is developed to address infections and manage iron metabolism in macrophages. This IME-responsive hydrogel, named FCL-ECMH, is synthesized by encapsulating vermiculite functional core layers within a decellularized extracellular matrix hydrogel. It is noteworthy that FCL-ECMH can produce reactive oxygen species within the IME. Supplementary photothermal treatment enhances bacterial iron uptake, leading to ferroptosis-like death. This process also rejuvenates the iron-enriched macrophages around the IME, thereby enhancing their antibacterial and tissue repair functions. In vivo experiments confirmed the antibacterial and repair-promoting capabilities of FCL-ECMH, indicating its potential for clinical applications.

中文翻译：

一种多功能组织工程水凝胶，旨在调节细菌铁死亡样死亡并克服感染以实现骨重塑

感染是骨科手术后最常见的并发症，可导致慢性伤口、骨缺损扩大和骨髓炎等长期疾病。铁对于细菌代谢和免疫细胞功能至关重要，极其重要。细菌利用附近细胞中的铁来促进生物膜的形成，确保它们的生存。因此，感染微环境 (IME) 内的铁缺乏会阻碍巨噬细胞功能，从而使感染进一步传播并阻碍巨噬细胞极化为 M2 表型。因此，提出了一种调节巨噬细胞极化的新方法，旨在恢复炎症免疫环境。开发了一种源自天然聚合物的复合水凝胶，用于解决感染和管理巨噬细胞中的铁代谢。这种 IME 响应性水凝胶名为 FCL-ECMH，是通过将蛭石功能核心层封装在脱细胞的细胞外基质水凝胶内而合成的。值得注意的是，FCL-ECMH 可以在 IME 内产生活性氧。补充光热治疗可增强细菌铁的吸收，导致铁死亡样死亡。这一过程还可以使 IME 周围的富铁巨噬细胞恢复活力，从而增强其抗菌和组织修复功能。体内实验证实了FCL-ECMH的抗菌和促进修复能力，表明其具有临床应用潜力。

更新日期：2024-06-19

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