Background

Hesperidin, a flavanone glycoside found primarily in the peel of citrus fruits, has benefits as a natural compound for preventing various diseases, but its inhibitory effect against oxidative injury in muscle cells has not been reported.

Objective

The current study aimed to investigate whether hesperidin can prevent oxidative damage in C2C12 murine myoblasts.

Results

Hesperidin was able to inhibit cytotoxicity while blocking hydrogen peroxide (H₂O₂) H₂O₂-induced DNA damage and apoptosis. Hesperidin also significantly improved the antioxidant capacity of C2C12 cells exposed to H₂O₂ by suppressing cellular reactive oxygen species production and increasing glutathione level. Additionally, H₂O₂-induced mitochondrial dysfunction and endoplasmic reticulum (ER) stress were effectively attenuated in the presence of hesperidin. Moreover, hesperidin neutralized H₂O₂-induced calcium ion (Ca²⁺) overload in mitochondria and mitigated the expression of cytosolic Ca²⁺-dependent proteases.

Conclusion

These results imply that hesperidin protects against mitochondrial impairment and Ca²⁺-mediated ER stress by minimizing oxidative stress, thereby suppressing H₂O₂-induced cytotoxicity in C2C12 myoblasts.

中文翻译：

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橙皮苷通过减少 ROS 介导的线粒体损伤和内质网应激，保护 C2C12 成肌细胞免受氧化损伤

 背景

橙皮苷是一种黄烷酮糖苷，主要存在于柑橘类水果的果皮中，作为天然化合物具有预防多种疾病的功效，但其对肌肉细胞氧化损伤的抑制作用尚未见报道。

 客观的

本研究旨在探讨橙皮苷是否可以预防 C2C12 小鼠成肌细胞的氧化损伤。

 结果

橙皮苷能够抑制细胞毒性，同时阻断过氧化氢 (H ₂ O ₂ ) H ₂ O ₂ 诱导的 DNA 损伤和细胞凋亡。橙皮苷还通过抑制细胞活性氧的产生和增加谷胱甘肽水平，显着提高暴露于 H ₂ O ₂ 的 C2C12 细胞的抗氧化能力。此外，在橙皮苷的存在下，H ₂ O ₂ 诱导的线粒体功能障碍和内质网（ER）应激得到有效减弱。此外，橙皮苷中和了 H ₂ O ₂ 诱导的线粒体钙离子 (Ca ²⁺ ) 超载，并减轻了胞质 Ca ²⁺ 依赖性蛋白酶。

 结论

这些结果表明，橙皮苷通过最大限度地减少氧化应激，从而抑制 H ₂ O ₂ 诱导的 C2C12 细胞毒性，从而防止线粒体损伤和 Ca ²⁺ 介导的 ER 应激。成肌细胞。