There are 13 known chicken blood systems, which were originally detected by agglutination of red blood cells by specific alloantisera. The genomic region or specific gene responsible has been identified for four of these systems (A, B, D and E). We determined the identity of the gene responsible for the chicken blood system I, using DNA from multiple birds with known chicken I blood system serology, 600K and 54K single nucleotide polymorphism (SNP) data, and lowpass sequence information. The gene responsible for the chicken I blood system was identified as RHCE, which is also one of the genes responsible for the highly polymorphic human Rh blood group locus, for which maternal/fetal antigenic differences can result in fetal hemolytic anemia with fetal mortality. We identified 17 unique RHCE haplotypes in the chicken, with six haplotypes corresponding to known I system serological alleles. We also detected deletions in the RHCE gene that encompass more than 6000 bp and that are predicted to remove its last seven exons. RHCE is the gene responsible for the chicken I blood system. This is the fifth chicken blood system for which the responsible gene and gene variants are known. With rapid DNA-based testing now available, the impact of I blood system variation on response against disease, general immune function, and animal production can be investigated in greater detail.

中文翻译：

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RHCE基因编码鸡血系统I


已知的鸡血系统有 13 种，最初是通过特定同种抗血清凝集红细胞来检测的。已确定其中四个系统（A、B、D 和 E）的基因组区域或特定基因。我们使用来自具有已知鸡血系统 I 血清学的多只鸡的 DNA、600K 和 54K 单核苷酸多态性 (SNP) 数据以及低通序列信息，确定了负责鸡血系统 I 的基因的身份。负责鸡I血液系统的基因被鉴定为RHCE，它也是负责高度多态性的人类Rh血型位点的基因之一，母体/胎儿抗原差异可导致胎儿溶血性贫血并导致胎儿死亡。我们在鸡中鉴定出 17 种独特的 RHCE 单倍型，其中 6 种单倍型对应于已知的 I 系统血清学等位基因。我们还检测到 RHCE 基因中包含超过 6000 bp 的缺失，预计会删除其最后 7 个外显子。 RHCE 是负责鸡 I 血液系统的基因。这是已知其相关基因和基因变异的第五个鸡血系统。现在有了基于 DNA 的快速测试，可以更详细地研究 I 血液系统变异对疾病反应、一般免疫功能和动物生产的影响。