Human trophectoderm becomes multi-layered by internalization at the polar region,Developmental Cell

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Human trophectoderm becomes multi-layered by internalization at the polar region
Developmental Cell ( IF 10.7 ) Pub Date : 2024-06-17 , DOI: 10.1016/j.devcel.2024.05.028
Elena Corujo-Simon ₁ , Lawrence Edward Bates ₁ , Ayaka Yanagida ₁ , Kenneth Jones ₁ , Stephen Clark ₂ , Ferdinand von Meyenn ₂ , Wolf Reik ₂ , Jennifer Nichols ₃

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To implant in the uterus, mammalian embryos form blastocysts comprising trophectoderm (TE) surrounding an inner cell mass (ICM), confined to the polar region by the expanding blastocoel. The mode of implantation varies between species. Murine embryos maintain a single layered TE until they implant in the characteristic thick deciduum, whereas human blastocysts attach via polar TE directly to the uterine wall. Using immunofluorescence (IF) of rapidly isolated ICMs, blockade of RNA and protein synthesis in whole embryos, or 3D visualization of immunostained embryos, we provide evidence of multi-layering in human polar TE before implantation. This may be required for rapid uterine invasion to secure the developing human embryo and initiate formation of the placenta. Using sequential fluorescent labeling, we demonstrate that the majority of inner TE in human blastocysts arises from existing outer cells, with no evidence of conversion from the ICM in the context of the intact embryo.

中文翻译：

人类滋养外胚层通过在极区内化而变得多层

为了植入子宫，哺乳动物胚胎形成囊胚，囊胚由滋养外胚层（TE）组成，围绕着内细胞团（ICM），被膨胀的囊胚腔限制在极地区域。植入方式因物种而异。小鼠胚胎保持单层 TE，直到它们植入特征性的厚蜕膜，而人类囊胚通过极性 TE 直接连接到子宫壁。使用快速分离的 ICM 的免疫荧光（IF）、阻断全胚胎中的 RNA 和蛋白质合成或免疫染色胚胎的 3D 可视化，我们提供了植入前人类极性 TE 多层的证据。这可能是快速子宫侵袭所必需的，以保护发育中的人类胚胎并开始胎盘的形成。使用连续荧光标记，我们证明人类囊胚中的大多数内部 TE 来自现有的外部细胞，没有证据表明在完整胚胎的背景下从 ICM 转换。

更新日期：2024-06-17

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