With the proposal of the 2030 Agenda for Sustainable Development, the Chinese medicine extraction technology has been innovatively improved to prioritize low energy consumption, sustainability, and minimized organic solvent utilization. (FS) possesses favorable pharmacological properties and is extensively utilized in traditional Chinese medicine. However, due to the limitations of the composition and extraction methods, its potential has not been fully developed. Thus, a combination of ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), and β-cyclodextrin extraction (β-CDE) was employed to isolate and purify rutin, phillyrin, and forsythoside A from FS. The results demonstrated that the efficiency of extracting enzymatic and ultrasound assisted β-cyclodextrin extraction (EUA-β-CDE) was highly influenced by the temperature and duration of hydrolysis, as well as the duration of the extraction process. According to the results of the single-factor experiment, Box-Behnken design (BBD) in Response surface method (RSM) was used to optimize the experimental parameters to achieve the maximum comprehensive evaluation value (CEV) value. The EUA-β-CDE compared with other extraction methods, has good extraction effect and low energy consumption by high performance liquid chromatography (HPLC), scanning electron microscopy (SEM), calculation of power consumption and CO emission The EUA-β-CDE compared with other extraction methods, has good extraction effect and low energy consumption by HPLC, SEM, calculation of power consumption and CO emission. Then, the structural characteristics of EUA-β-CDE of FS extract had significant interaction with β-CD by Fourier infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). In addition, EUA-β-CDE extract has good antioxidant and anti-inflammatory activities. The establishment of EUA-β-CDE of FS provides a new idea for the development and application of other sustainable extraction methods of traditional Chinese medicine.

中文翻译：

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酶法和超声辅助β-环糊精提取连翘活性成分及其抗氧化和抗炎活性


随着2030年可持续发展议程的提出，中药提取技术得到创新改进，优先考虑低能耗、可持续、尽量减少有机溶剂的使用。 (FS)具有良好的药理特性，在中药中广泛使用。但由于成分和提取方法的限制，其潜力尚未得到充分开发。因此，采用超声辅助提取（UAE）、酶辅助提取（EAE）和β-环糊精提取（β-CDE）相结合的方法从FS中分离和纯化芦丁、连翘苷和连翘苷A。结果表明，酶法和超声辅助β-环糊精提取（EUA-β-CDE）的提取效率很大程度上受水解温度和持续时间以及提取过程持续时间的影响。根据单因素实验结果，采用响应面法（RSM）中的Box-Behnken设计（BBD）对实验参数进行优化，以达到综合评价值（CEV）值最大。通过高效液相色谱（HPLC）、扫描电镜（SEM）、计算电耗和CO排放量，得出EUA-β-CDE与其他提取方法相比，提取效果好，能耗低。通过HPLC、SEM、计算电耗和CO排放量，与其他提取方法相比，提取效果好，能耗低。然后通过傅里叶红外光谱（FT-IR）和差示扫描量热法（DSC）发现FS提取物的EUA-β-CDE的结构特征与β-CD具有显着的相互作用。 此外，EUA-β-CDE提取物具有良好的抗氧化和抗炎活性。 FS EUA-β-CDE的建立为其他中药可持续提取方法的开发和应用提供了新思路。