leaf polysaccharide (AALPs) were prepared through ultrasound-assisted extraction (UAE), and their antifatigue activities were evaluated. Extraction was optimized using response surface methodology (RSM), which yielded the following optimal UAE conditions: ultrasonication power of 300 W, extraction temperature of 51 °C, liquid:solid ratio of 20 mL/g, and ultrasonication time of 47 mins. The above optimal conditions resulted in the maximum extraction rate of 10.49 %. Compared with hot water extraction (HWE), UAE supported higher yields and total sugar, uronic acid, and sulfate contents of AALPs. Meanwhile, AALP prepared through UAE (AALP-U) exhibited higher stability due to its smaller particle size and higher absolute value of zeta potential than AALP prepared through HWE (AALP-H). In addition, AALP-U demonstrated stronger antioxidant activity than AALP-H. In forced swimming tests on mice, AALP-U could significantly prolong swimming time with a dose-dependent effect, increase liver and muscle glycogen levels, and improve other biochemical indices, thus showing great potential for application in functional food.

中文翻译：

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艾叶多糖：环保超声辅助提取和抗疲劳活性


通过超声辅助提取（UAE）制备叶多糖（AALPs），并评估其抗疲劳活性。采用响应面法 (RSM) 优化提取，得到以下最佳阿联酋条件：超声处理功率为 300 W，提取温度为 51 °C，液固比为 20 mL/g，超声处理时间为 47 分钟。上述最佳条件下的最大提取率为 10.49%。与热水提取 (HWE) 相比，UAE 具有更高的产量以及 AALP 的总糖、糖醛酸和硫酸盐含量。同时，与通过HWE制备的AALP（AALP-H）相比，通过UAE制备的AALP（AALP-U）由于其更小的粒径和更高的zeta电位绝对值而表现出更高的稳定性。此外，AALP-U 表现出比 AALP-H 更强的抗氧化活性。在小鼠强迫游泳试验中，AALP-U可显着延长游泳时间，且呈剂量依赖性效应，提高肝糖原和肌糖原水平，改善其他生化指标，在功能食品中显示出巨大的应用潜力。