Discovery of petroleum ether extract of eclipta targeting p53/Fas pathway for the treatment of chemotherapy-induced alopecia: Network pharmacology and experimental validation,Journal of Ethnopharmacology

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Discovery of petroleum ether extract of eclipta targeting p53/Fas pathway for the treatment of chemotherapy-induced alopecia: Network pharmacology and experimental validation
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2024-06-04 , DOI: 10.1016/j.jep.2024.118405
Wuji Wang ₁ , Honglan Wang ₁ , Yang Luo ₁ , Zheng Li ₁ , Jingjie Li ₁

Affiliation

Ecliptea herba, a traditional Chinese herbal medicine for hair loss, was first recorded in the Tang Dynasty's ‘Qian Jin Yue Ling’, of which the active ingredients and mechanisms of action in the treatment of chemotherapy-induced hair loss remain poorly investigated. To investigate the effects of the petroleum ether extract of Eclipta (PEE) on alopecia and follicle damage and elucidate its potential therapeutic mechanisms using the integration of network pharmacology, bioinformatics, and experimental validation. UPLC-MS was used to analyse the chemical composition of PEE. A network pharmacology approach was employed to establish the ‘components-targets-pathways’ network of PEE to explore potential therapeutic pathways and targets. Molecular docking was used for validation, and the mechanism of PEE in treating chemotherapy-induced alopecia (CIA) was elucidated using and on CIA models. UPLC-MS analysis of PEE revealed 185 components, while network pharmacology and molecular docking analyses revealed potential active compounds and their target molecules, suggesting the involvement of core genes, such as , , , , , and . The key components included wedelolactone, dimethyl-wedelolactone, luteoloside, linarin, and hispidulin. , PEE promoted hair growth, restored the number of hair follicles, and reduced follicle apoptosis. Conversely, , PEE enhanced cell viability, reduced apoptosis, and protected HaCaT cells from damage induced by 4-hydroperoxycyclophosphamide (4-HC). PEE alleviated hair follicle damage in CIA mice by inhibiting the P53/Fas pathway, which may be associated with inhibiting hair follicle cell apoptosis. This study provides a novel therapeutic strategy for treating cyclophosphamide-induced hair loss.

中文翻译：

发现旱莲草石油醚提取物靶向 p53/Fas 通路治疗化疗引起的脱发：网络药理学和实验验证

旱莲草是治疗脱发的传统中草药，最早记载于唐代的《千金越令》中，但其治疗化疗引起的脱发的有效成分和作用机制尚不清楚。结合网络药理学、生物信息学和实验验证，研究旱莲草石油醚提取物 (PEE) 对脱发和毛囊损伤的影响，并阐明其潜在的治疗机制。采用UPLC-MS分析PEE的化学成分。采用网络药理学方法建立PEE的“成分-靶点-途径”网络，以探索潜在的治疗途径和靶点。采用分子对接进行验证，并在 CIA 模型上阐明了 PEE 治疗化疗引起的脱发 (CIA) 的机制。 PEE 的 UPLC-MS 分析揭示了 185 个成分，而网络药理学和分子对接分析揭示了潜在的活性化合物及其靶分子，表明核心基因的参与，例如、、、、和。主要成分包括蟛蜞菊内酯、二甲基-蟛蜞菊内酯、木犀草苷、里那苷和刚毛素。、PEE促进毛发生长，恢复毛囊数量，减少毛囊凋亡。相反，PEE 增强细胞活力，减少细胞凋亡，并保护 HaCaT 细胞免受 4-氢过氧环磷酰胺 (4-HC) 诱导的损伤。 PEE通过抑制P53/Fas通路减轻CIA小鼠的毛囊损伤，这可能与抑制毛囊细胞凋亡有关。这项研究为治疗环磷酰胺引起的脱发提供了一种新的治疗策略。

更新日期：2024-06-04

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