Inflammation is an innate defense response of an organism to tissue damage which is accompanied by biological processes such as depolarization, autophagy, and endogenous bisulfite production. As a novel type of iron-dependent programmed apoptosis, ferroptosis accumulates an excessive lipid peroxide, leading to fluctuations of polarity and SO. To study the dynamic correlation between SO and polarity of these two diseases is of great significance for revealing the precise regulation mechanism of inflammation and ferroptosis. In this research, a multicolor fluorescent probe was developed by coupling a sulfur dioxide response site with push-pull electrons, realizing orange and near-infrared fluorescence emission, allowing to the simultaneous detection of polarity and SO. With its attractive AIE and NIR properties, is able to effectively prevent background interference and effectively solve the problem of fluorescence quenching. For the first time, the slight fluctuations in SO and polarity during inflammation and ferroptosis is shown by examining parameter changes during aberrant processes at the and in vitro levels. This offers fresh insights into the diagnosis and therapy of disease processes.

中文翻译：

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三通道荧光探针观察炎症和铁死亡的诊断和治疗过程


炎症是生物体对组织损伤的先天防御反应，伴随着去极化、自噬和内源性亚硫酸氢盐产生等生物过程。作为一种新型的铁依赖性程序性细胞凋亡，铁死亡会积累过量的脂质过氧化物，导致极性和 SO 的波动。研究SO与两种疾病极性的动态相关性对于揭示炎症和铁死亡的精准调控机制具有重要意义。本研究通过将二氧化硫响应位点与推挽电子耦合，开发了多色荧光探针，实现了橙色和近红外荧光发射，从而可以同时检测极性和SO。凭借其极具吸引力的AIE和NIR特性，能够有效防止背景干扰并有效解决荧光猝灭问题。通过检查体内和体外水平异常过程中的参数变化，首次显示了炎症和铁死亡期间 SO 和极性的轻微波动。这为疾病过程的诊断和治疗提供了新的见解。