Guss., a species belonging to the Apiaceae family, is an exclusive and endemic plant of the Sicily region, Italy. Considering the limited scientific attention given to , herein, for the first time, metabolite profiles of polar extracts (MeOH) of the aerial parts, flowers, and roots were investigated by liquid chromatography coupled to electrospray ionization and high resolution multiple-stage mass spectrometry (LC-ESI/HRMSn), allowing the identification of 32 compounds, never identified in . Metabolites belonging to the classes of organic acids, megastigmanes, phenylpropanoids, iridoids, flavonoids, coumarins, triterpenoids, and polar lipids were putatively identified; in particular, several compoundsare reported here for the first time in genus. Considering the increasing interest for the development of botanical insecticides, the acaricidal activity of against the two-spotted spider mite Koch was evaluated. Although all extracts showed promising acaricidal activity, the most effective was the extract obtained from the aerial parts. The lowest LD and LD values of 2.3 and 77.3 μg‧cm were estimated for this extract from aerial parts, although this LD50 was statistically identical to the LD of the roots extract. The extract from aerial parts not only caused the mortality of adults, but also inhibited the fertility and fecundity of females, even when a dose of 5 μg‧cm was applied. Based on our experiments, MeOH extracts from aerial parts biomass of can be proposed as a perspective source of active substances of potential botanical acaricides.

中文翻译：

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Ammi crintum Guss 的植物化学研究和杀螨活性评价。防治二斑叶螨 二点叶螨 (Tetranychus urticae Koch)


Guss. 属于伞形科植物，是意大利西西里岛地区特有的特有植物。考虑到科学关注有限，本文首次通过液相色谱结合电喷雾电离和高分辨率多级质谱研究了地上部分、花和根的极性提取物（MeOH）的代谢物谱。 LC-ESI/HRMSn)，允许鉴定 32 种化合物，这些化合物从未在 .推测鉴定出属于有机酸、巨豆碱、苯丙素、环烯醚萜类、黄酮类、香豆素、三萜类和极性脂质类的代谢物；特别是，一些化合物是首次在属中报道。考虑到人们对植物源杀虫剂开发的兴趣日益浓厚，对二斑叶螨Koch的杀螨活性进行了评估。尽管所有提取物均显示出良好的杀螨活性，但最有效的是从地上部分获得的提取物。尽管此 LD50 在统计上与根提取物的 LD 相同，但估计该地上部分提取物的最低 LD 和 LD 值分别为 2.3 和 77.3 µg·cm。地上部分提取物不仅会导致成虫死亡，而且即使施用剂量为5μg·cm，也会抑制雌性的生育力和繁殖力。根据我们的实验，从地上部分生物质中提取的甲醇可以作为潜在植物杀螨剂活性物质的潜在来源。