1,3-Propanediol (PDO) is a value-added chemical used widely in cosmetics, foods, lubricants, adhesives, and pharmaceuticals. is a facultative anaerobe that can produce 1,3-PDO through the fermentation pathway of glycerol. Recently, formate produced (bio)electrochemically from CO has been highlighted as an intermediary metabolite and environmentally friendly biological reducing agent for bioconversion. This study examined how the glycerol fermentation of with various formate concentrations enhances 1,3-PDO production. Glycerol consumption increased from 58.7 % to 92.6 %, while the 1,3-PDO titer was improved simultaneously from 172.5 to 251.0 mM using 50 mM of formate. During 1,3-PDO production, the NADH pool increased to 345.3 nM/OD, 2.4 times higher than the control. Therefore, adding formate to glycerol fermentation reduces the residual glycerol and increases 1,3-PDO production simultaneously, which can be an alternative bioconversion strategy without genetic modification.

中文翻译：

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使用甲酸盐作为产酸克雷伯氏菌中 NADH 再生的中间代谢物改善甘油生产 1,3-丙二醇


1,3-丙二醇 (PDO) 是一种增值化学品，广泛用于化妆品、食品、润滑剂、粘合剂和药品。是一种兼性厌氧菌，可通过甘油发酵途径产生1,3-PDO。最近，由CO（生物）电化学产生的甲酸被认为是一种中间代谢物和用于生物转化的环境友好型生物还原剂。本研究探讨了不同甲酸浓度的甘油发酵如何提高 1,3-PDO 的产量。使用 50 mM 甲酸盐，甘油消耗量从 58.7% 增加到 92.6%，同时 1,3-PDO 滴度从 172.5 mM 提高到 251.0 mM。在 1,3-PDO 生产过程中，NADH 池增加至 345.3 nM/OD，是对照的 2.4 倍。因此，在甘油发酵中添加甲酸可以减少残留甘油，同时增加1,3-PDO产量，这可以成为一种无需基因改造的替代生物转化策略。