Monitoring the levels of L-Tryptophan (L-Trp) in body fluids is crucial due to its significant role in metabolism and protein synthesis, which ultimately affects neurological health. Herein, we have developed a novel magneto-responsive electrochemical enantioselective sensor for the recognition of L-Trp based on oriented biochar derived from Loofah, FeO nanoparticles, and molecularly imprinted polydopamine (MIPDA) in xanthan hydrogel. The successful synthesis of these materials has been confirmed through physicochemical and electrochemical characterization. Various operational factors such as pH, response time, loading sample volume, and loading of active materials were optimized. As a result, the sensor exhibited an affordable linear range of 1.0–60.0 μM, with a desirable limit of detection of 0.44 μM. Furthermore, the proposed electrochemical sensor demonstrated good reproducibility and desirable selectivity for the determination of L-Trp, making it suitable for analyzing L-Trp levels in human plasma and serum samples. The development presented offers an appealing, easily accessible, and efficient strategy. It utilizes xanthan hydrogel to improve mass transfer and adhesion, biochar-stabilized FeO to facilitate magnetic orientation and accelerate mass transfer and sensitivity, and polydopamine MIP to enhance selectivity. This approach enables on-site evaluation of L-Trp levels, which holds significant value for healthcare monitoring and early detection of related conditions.

中文翻译：

---

远程磁刺激黄原胶-生物炭-Fe3O4-分子印迹生物聚合物水凝胶用于 L-色氨酸的电化学对映选择


监测体液中 L-色氨酸 (L-Trp) 的水平至关重要，因为它在新陈代谢和蛋白质合成中发挥着重要作用，最终影响神经健康。在此，我们开发了一种新型磁响应电化学对映选择性传感器，用于识别基于丝瓜络定向生物炭、Fe3O纳米颗粒和黄原胶水凝胶中的分子印迹聚多巴胺（MIPDA）的L-Trp。这些材料的成功合成已通过物理化学和电化学表征得到证实。各种操作因素（例如 pH、响应时间、加载样品体积和活性材料的加载）都得到了优化。因此，该传感器表现出 1.0–60.0 μM 的线性范围，理想的检测限为 0.44 μM。此外，所提出的电化学传感器在 L-Trp 测定方面表现出良好的重现性和理想的选择性，使其适合分析人血浆和血清样品中的 L-Trp 水平。所呈现的开发提供了一个有吸引力的、易于访问且高效的策略。它利用黄原胶水凝胶来改善传质和粘附，利用生物炭稳定的 Fe3O 来促进磁性取向并加速传质和灵敏度，并利用聚多巴胺 MIP 来增强选择性。这种方法可以现场评估 L-Trp 水平，这对于医疗保健监测和相关病症的早期检测具有重要价值。