In recent years, miRNAs have emerged as potentially valuable tumor markers, and their sensitive and accurate detection is crucial for early screening and diagnosis of tumors. However, the analysis of miRNAs faces significant challenges due to their short sequence, susceptibility to degradation, high similarity, low expression level in cells, and stringent requirements for in vitro research environments. Therefore, the development of sensitive and efficient new methods for the detection of tumor markers is crucial for the early intervention of related tumors. An ultrasensitive electrochemical/colorimetric dual-mode self-powered biosensor platform is established to detect microRNA-21 (miR-21) via a multi-signal amplification strategy. Gold nanoparticles (AuNPs) and VS nanosheets self-assembled 3D nanorods (VS-Ns-Nrs) are prepared for constructing a superior performance enzyme biofuel cell (EBFC). The double-signal amplification strategy of Y-shaped DNA nanostructure and catalytic hairpin assembly (CHA) is adopted to further improve enhance the strength and specificity of the output signal. In addition, a capacitor is matched with EBFC to generate an instantaneous current that is amplified several times, and the output detection signal is improved once more. At the same time, electrochemical and colorimetric methods are used for dual-mode strategy to achieve the accuracy of detection. The linear range of detection is from 0.001 pg/mL to 1000 pg/mL, with a relatively low limit of detection (LOD) of 0.16 fg/mL (S/N = 3). The established method enables accurate and sensitive detection of markers in patients with lung cancer, providing technical support and data reference for precise identification. It is anticipated to offer a sensitive and practical new technology and approach for early diagnosis, clinical treatment, and drug screening of cancer and other related major diseases.

中文翻译：

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超灵敏电化学/比色双模式自供电生物传感平台，通过多信号放大策略检测肺癌标志物


近年来，miRNA已成为潜在有价值的肿瘤标志物，其灵敏、准确的检测对于肿瘤的早期筛查和诊断至关重要。然而，由于miRNA序列短、易降解、相似性高、细胞内表达水平低以及对体外研究环境的严格要求，miRNA的分析面临着巨大的挑战。因此，开发灵敏、高效的肿瘤标志物检测新方法对于相关肿瘤的早期干预至关重要。建立了超灵敏电化学/比色双模式自供电生物传感器平台，通过多信号放大策略检测 microRNA-21 (miR-21)。制备金纳米粒子（AuNPs）和VS纳米片自组装3D纳米棒（VS-Ns-Nrs）用于构建高性能酶生物燃料电池（EBFC）。采用Y型DNA纳米结构和催化发夹组装（CHA）的双信号放大策略，进一步提高输出信号的强度和特异性。另外，电容与EBFC配合产生数倍放大的瞬时电流，输出检测信号再次得到改善。同时采用电化学和比色法双模式策略，实现检测的准确性。检测的线性范围为 0.001 pg/mL 至 1000 pg/mL，检测限 (LOD) 相对较低，为 0.16 fg/mL (S/N = 3)。所建立的方法能够准确、灵敏地检测肺癌患者的标志物，为精准识别提供技术支持和数据参考。 有望为癌症及其他相关重大疾病的早期诊断、临床治疗和药物筛选提供灵敏、实用的新技术和新方法。