In the immunoassay process, for fulfilling the need to identify multiple analytes in a small amount of complex sample matrix, it is desirable to develop highly efficient and specific multiplex suspension array technology. Raman coding strategy offers an attractive solution to code the suspension arrays by simply combing narrow spectral bands with stable signal intensities through solid-phase synthesis on the resin beads. Based on this strategy, we report the bead-based spontaneous Raman codes for multiplex immunoassay. The study resulted in superior selectivity of the Raman-encoded beads for binding with single and multiple analytes, respectively. With the use of mixed types of Raman-encoded immunoassay beads, multiple targets in small amounts of samples were identified rapidly and accurately. By confirming the feasibility of bead-based spontaneous Raman codes for multiplex immunoassay, we anticipate this novel technology to be widely applied in the near future.

中文翻译：

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用于多重免疫分析的基于微珠的自发拉曼编码


在免疫分析过程中，为了满足在少量复杂样品基质中鉴定多种分析物的需要，需要开发高效且特异的多重悬浮阵列技术。拉曼编码策略提供了一种有吸引力的解决方案，通过在树脂珠上进行固相合成，简单地将窄光谱带与稳定的信号强度结合起来，对悬浮阵列进行编码。基于这一策略，我们报告了用于多重免疫分析的基于珠子的自发拉曼代码。该研究表明拉曼编码珠分别与单一和多种分析物结合具有优异的选择性。通过使用混合类型的拉曼编码免疫分析珠，可以快速准确地识别少量样品中的多个目标。通过确认基于微珠的自发拉曼编码用于多重免疫分析的可行性，我们预计这项新技术将在不久的将来得到广泛应用。