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IVF exposure induced intergenerational effects on metabolic phenotype in mice
Reproductive BioMedicine Online ( IF 3.7 ) Pub Date : 2024-04-13 , DOI: 10.1016/j.rbmo.2024.103992 Miaomiao Ban 1 , Wanbing Feng 1 , Min Hou 1 , Zhirong Zhang 1 , Linlin Cui 2
Reproductive BioMedicine Online ( IF 3.7 ) Pub Date : 2024-04-13 , DOI: 10.1016/j.rbmo.2024.103992 Miaomiao Ban 1 , Wanbing Feng 1 , Min Hou 1 , Zhirong Zhang 1 , Linlin Cui 2
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What is the potential transmission of metabolic phenotype from IVF offspring to the subsequent generation? An IVF mouse model was established. The F generation mice were produced though IVF or natural mating and the F generation was obtained through the mating of F generation males with normal females. Their metabolic phenotype, including systemic and hepatic glucolipid metabolism, was examined. It was found that IVF F males exhibited metabolic changes. Compared with the control group, the IVF F generation showed increased body weight, elevated fasting glucose and insulin, and increased serum triglyceride concentrations. IVF F mice also showed an increased expression of hepatic lipogenesis and autophagy genes. Moreover, IVF F males transmitted some metabolic changes to their own male progeny (IVF F) in the absence of a dietary challenge. IVF F mice had increased peri-epididymal and subcutaneous fat and decreased insulin sensitivity. Under the ‘second hit’ of a high-fat diet, IVF F mice further showed increased hepatic lipid deposition with unaltered autophagy levels. This research demonstrates the impact of IVF on hepatic glucose–lipid metabolism in two successive generations of offspring, highlighting the need for additional investigation. Enhanced understanding of the mechanisms underlying the transmission of multigenerational effects induced by IVF could potentially lead to the advancement of therapeutic interventions for individuals experiencing infertility.
中文翻译：
体外受精暴露对小鼠代谢表型产生代际影响
IVF 后代的代谢表型向下一代的潜在传递是什么？建立IVF小鼠模型。 F代小鼠通过体外受精或自然交配产生，F代小鼠是通过F代雄性与正常雌性交配获得的。检查了他们的代谢表型，包括全身和肝脏糖脂代谢。结果发现，IVF F 雄性表现出代谢变化。与对照组相比，IVF F代表现出体重增加、空腹血糖和胰岛素升高、血清甘油三酯浓度升高。 IVF F 小鼠还表现出肝脏脂肪生成和自噬基因表达增加。此外，在没有饮食挑战的情况下，IVF F 雄性将一些代谢变化传递给自己的雄性后代 (IVF F)。 IVF F小鼠的附睾周围和皮下脂肪增加，胰岛素敏感性降低。在高脂肪饮食的“第二次打击”下，IVF F 小鼠进一步表现出肝脏脂质沉积增加，而自噬水平未改变。这项研究证明了体外受精对连续两代后代的肝脏糖脂代谢的影响，强调了进一步研究的必要性。加深对体外受精引起的多代效应传递机制的理解可能会促进针对不孕症患者的治疗干预措施的进步。
更新日期：2024-04-13
中文翻译：
体外受精暴露对小鼠代谢表型产生代际影响
IVF 后代的代谢表型向下一代的潜在传递是什么？建立IVF小鼠模型。 F代小鼠通过体外受精或自然交配产生，F代小鼠是通过F代雄性与正常雌性交配获得的。检查了他们的代谢表型，包括全身和肝脏糖脂代谢。结果发现，IVF F 雄性表现出代谢变化。与对照组相比，IVF F代表现出体重增加、空腹血糖和胰岛素升高、血清甘油三酯浓度升高。 IVF F 小鼠还表现出肝脏脂肪生成和自噬基因表达增加。此外，在没有饮食挑战的情况下，IVF F 雄性将一些代谢变化传递给自己的雄性后代 (IVF F)。 IVF F小鼠的附睾周围和皮下脂肪增加，胰岛素敏感性降低。在高脂肪饮食的“第二次打击”下，IVF F 小鼠进一步表现出肝脏脂质沉积增加，而自噬水平未改变。这项研究证明了体外受精对连续两代后代的肝脏糖脂代谢的影响，强调了进一步研究的必要性。加深对体外受精引起的多代效应传递机制的理解可能会促进针对不孕症患者的治疗干预措施的进步。
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