Wound infection of hyperglycemic patient often has extended healing period and increased probability due to the high glucose level. However, achieving precise and safe therapy of the hyperglycemic wound with specific wound microenvironment (WME) remains a major challenge. Herein, a WME-activated smart L-Arg/GOx@TA-Fe (LGTF) nanozymatic system composed of generally recognized as safe (GRAS) compound is engineered. The nanozymatic system combining metal-polyphenol nanozyme (tannic acid-Fe, TA-Fe) and natural enzyme (glucose oxidase, GOx) can consume the high-concentration glucose, generating reactive oxygen species (ROS) and nitric oxide (NO) in situ to synergistically disinfect hyperglycemia wound. In addition, glucose consumption and gluconic acid generation can lower glucose level to promote wound healing and reduce the pH of WME to enhance the catalytic activities of the LGTF nanozymatic system. Thereby, low-dose LGTF can perform remarkable synergistic disinfection and healing effect towards hyperglycemic wound. The superior biosafety, high catalytic antibacterial and beneficial WME regulating capacity demonstrate this benign GRAS nanozymatic system is a promising therapeutic agent for hyperglycemic wound.

中文翻译：

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微环境触发级联金属多酚纳米酶用于ROS/NO协同高血糖伤口愈合


高血糖患者的伤口感染往往因血糖水平高而延长愈合时间并增加可能性。然而，在特定伤口微环境（WME）下实现高血糖伤口的精确、安全治疗仍然是一个重大挑战。在此，设计了一种 WME 激活的智能 L-Arg/GOx@TA-Fe (LGTF) 纳米酶系统，该系统由公认的安全 (GRAS) 化合物组成。金属多酚纳米酶（单宁酸-Fe，TA-Fe）和天然酶（葡萄糖氧化酶，GOx）相结合的纳米酶系统可以消耗高浓度的葡萄糖，原位产生活性氧（ROS）和一氧化氮（NO）协同消毒高血糖伤口。此外，葡萄糖消耗和葡萄糖酸生成可以降低葡萄糖水平以促进伤口愈合，并降低WME的pH以增强LGTF纳米酶系统的催化活性。由此可见，低剂量LGTF对高血糖伤口具有显着的协同消毒和愈合作用。卓越的生物安全性、高催化抗菌性和有益的 WME 调节能力表明这种良性 GRAS 纳米酶系统是一种有前途的高血糖伤口治疗剂。