Endophytic fungi associated with plants may contain undiscovered bioactive compounds. Under standard laboratory conditions, most undiscovered compounds are inactive, whereas their production could be stimulated under different cultivation conditions. In this study, six endophytic fungi were isolated from the bark of Koelreuteria paniculata in Quancheng Park, Jinan City, Shandong Province, one of which was identified as a new subspecies of Aureobasidium pullulans, named A. pullulans KB3. Additionally, metabolomic tools were used to screen suitable media for A. pullulans KB3 fermentation, and the results showed that peptone dextrose medium (PDM) was more beneficial to culture A. pullulans KB3 for isolation of novel compounds. Sphaerolone, a polyketone compound, was initially isolated from A. pullulans KB3 via scaled up fermentation utilizing PDM. Additionally, the whole-genome DNA of A. pullulans KB3 was sequenced to facilitate compound isolation and identify the biosynthesis gene clusters (BGCs). This study reports the multi-omics (metabolome and genome) analysis of A. pullulans KB3, laying the foundation for discovering novel compounds of silent BGCs and identifying their biosynthesis pathway.

与植物相关的内生真菌可能含有未被发现的生物活性化合物。在标准实验室条件下，大多数未发现的化合物都是无活性的，而它们的产生可以在不同的培养条件下被刺激。本研究从山东省济南市泉城公园栾树树皮中分离得到6种内生真菌，其中1种经鉴定为出芽短梗霉新亚种，命名为出芽短梗霉KB3。此外，利用代谢组学工具筛选适合出芽短梗霉KB3发酵的培养基，结果表明蛋白胨葡萄糖培养基（PDM）更有利于培养出芽短梗霉KB3以分离新化合物。 Sphaerolone 是一种聚酮化合物，最初是利用 PDM 通过放大发酵从出芽短梗霉 KB3 中分离出来的。此外，还对出芽短梗霉KB3 的全基因组 DNA 进行了测序，以促进化合物分离并鉴定生物合成基因簇 (BGC)。本研究报告了出芽短梗霉KB3的多组学（代谢组和基因组）分析，为发现沉默BGC的新型化合物并确定其生物合成途径奠定了基础。