Lymphatic transport shapes the homeostatic immune repertoire of lymph nodes (LNs). LN-resident memory T cells (T RMs ) play an important role in site-specific immune memory, yet how LN T RMs form de novo after viral infection remains unclear. Here, we tracked the anatomical distribution of antiviral CD8 + T cells as they seeded skin and LN T RMs using a model of vaccinia virus–induced skin infection. LN T RMs localized to the draining LNs (dLNs) of infected skin, and their formation depended on the lymphatic egress of effector CD8 + T cells from the skin, already poised for residence. Effector CD8 + T cell transit through skin was required to populate LN T RMs in dLNs, a process reinforced by antigen encounter in skin. Furthermore, LN T RMs were protective against viral rechallenge in the absence of circulating memory T cells. These data suggest that a subset of tissue-infiltrating CD8 + T cells egress from tissues during viral clearance and establish a layer of regional protection in the dLN basin.

中文翻译：

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淋巴管转运在皮肤引流淋巴结中播种细胞毒性常驻记忆 T 细胞


淋巴运输塑造淋巴结 (LN) 的稳态免疫库。 LN 驻留记忆 T 细胞（T均方根）在位点特异性免疫记忆中发挥重要作用，但LN T如何均方根病毒感染后从头形成的情况仍不清楚。在这里，我们追踪了抗病毒 CD8 的解剖分布+ T 细胞接种皮肤和 LN T均方根使用痘苗病毒引起的皮肤感染模型。液氮均方根定位于受感染皮肤的引流淋巴结 (dLN)，其形成取决于效应器 CD8 的淋巴出口+来自皮肤的 T 细胞，已经做好了驻留的准备。效应器CD8 + T 细胞需要穿过皮肤来填充 LN T均方根在 dLN 中，这一过程因皮肤中的抗原相遇而加强。此外，LN T均方根在没有循环记忆 T 细胞的情况下，可以防止病毒再攻击。这些数据表明组织浸润 CD8 的一个子集+ T 细胞在病毒清除过程中从组织中逸出，并在 dLN 盆地中建立一层区域保护。