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Acoustofluidic-based microscopic examination for automated and point-of-care urinalysis
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-13 , DOI: 10.1039/d4lc00408f Xin He 1, 2 , Feng Ren 3 , Yangyang Wang 1, 2 , Zhiyuan Zhang 1, 2 , Jiming Zhou 1, 2 , Jian Huang 1, 2 , Shuye Cao 1, 2 , Jinying Dong 3 , Renxin Wang 4 , Mengxi Wu 1, 2 , Junshan Liu 1, 2
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-13 , DOI: 10.1039/d4lc00408f Xin He 1, 2 , Feng Ren 3 , Yangyang Wang 1, 2 , Zhiyuan Zhang 1, 2 , Jiming Zhou 1, 2 , Jian Huang 1, 2 , Shuye Cao 1, 2 , Jinying Dong 3 , Renxin Wang 4 , Mengxi Wu 1, 2 , Junshan Liu 1, 2
Affiliation
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Urinalysis is a heavily used diagnostic test in clinical laboratories; however, it is chronically held back by urine sediment microscopic examination. Current instruments are bulky and expensive to be widely adopted, making microscopic examination a procedure that still relies on manual operations and requires large time and labor costs. To improve the efficacy and automation of urinalysis, this study develops an acoustofluidic-based microscopic examination system. The system utilizes the combination of acoustofluidic manipulation and a passive hydrodynamic mechanism, and thus achieves a high throughput (1000 μL min−1) and a high concentration factor (95.2 ± 2.1 fold) simultaneously, fulfilling the demands for urine examination. The concentrated urine sample is automatically dispensed into a hemocytometer chamber and the images are then analyzed using a machine learning algorithm. The whole process is completed within 3 minutes with detection accuracies of erythrocytes and leukocytes of 94.6 ± 3.5% and 95.1 ± 1.8%, respectively. The examination outcome of urine samples from 50 volunteers by this device shows a correlation coefficient of 0.96 compared to manual microscopic examination. Our system offers a promising tool for automated urine microscopic examination, thus it has potential to save a large amount of time and labor in clinical laboratories, as well as to promote point-of-care urine testing applications in and beyond hospitals.
中文翻译：
基于声流控的显微镜检查，用于自动和护理点尿液分析
尿液分析是临床实验室中广泛使用的诊断测试；然而，尿沉渣显微镜检查却长期阻碍了它的发展。目前的仪器体积庞大且价格昂贵，难以广泛采用，使得显微镜检查仍然依赖于手动操作，并且需要大量的时间和劳动力成本。为了提高尿液分析的效率和自动化，本研究开发了一种基于声流控的显微镜检查系统。该系统利用声流控操作和被动流体动力机制的结合，从而同时实现高通量（1000 μL min -1 ）和高浓缩倍数（95.2 ± 2.1倍），满足尿液检查的要求。浓缩的尿液样本自动分配到血细胞计数器室中，然后使用机器学习算法分析图像。整个过程在3分钟内完成，红细胞和白细胞的检测准确率分别为94.6±3.5%和95.1±1.8%。该装置对 50 名志愿者尿液样本的检查结果显示，与手动显微镜检查相比，相关系数为 0.96。我们的系统为自动化尿液显微镜检查提供了一种很有前途的工具，因此它有可能节省临床实验室的大量时间和劳动力，并促进医院内外的即时尿液检测应用。
更新日期：2024-06-13
中文翻译：
基于声流控的显微镜检查，用于自动和护理点尿液分析
尿液分析是临床实验室中广泛使用的诊断测试；然而，尿沉渣显微镜检查却长期阻碍了它的发展。目前的仪器体积庞大且价格昂贵，难以广泛采用，使得显微镜检查仍然依赖于手动操作，并且需要大量的时间和劳动力成本。为了提高尿液分析的效率和自动化，本研究开发了一种基于声流控的显微镜检查系统。该系统利用声流控操作和被动流体动力机制的结合，从而同时实现高通量（1000 μL min -1 ）和高浓缩倍数（95.2 ± 2.1倍），满足尿液检查的要求。浓缩的尿液样本自动分配到血细胞计数器室中，然后使用机器学习算法分析图像。整个过程在3分钟内完成，红细胞和白细胞的检测准确率分别为94.6±3.5%和95.1±1.8%。该装置对 50 名志愿者尿液样本的检查结果显示，与手动显微镜检查相比，相关系数为 0.96。我们的系统为自动化尿液显微镜检查提供了一种很有前途的工具，因此它有可能节省临床实验室的大量时间和劳动力，并促进医院内外的即时尿液检测应用。
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