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Laser-induced microbubble as an in vivo valve for optofluidic manipulation in living Mice's microvessels
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-13 , DOI: 10.1039/d4lc00095a Meng Shao 1 , Changxu Li 2 , Chun Meng 1 , Rui Liu 2 , Panpan Yu 1 , Fengya Lu 2 , Zhensheng Zhong 2 , Xunbin Wei 2, 3 , Jinhua Zhou 2 , Min-Cheng Zhong 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-13 , DOI: 10.1039/d4lc00095a Meng Shao 1 , Changxu Li 2 , Chun Meng 1 , Rui Liu 2 , Panpan Yu 1 , Fengya Lu 2 , Zhensheng Zhong 2 , Xunbin Wei 2, 3 , Jinhua Zhou 2 , Min-Cheng Zhong 1
Affiliation
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Optofluidic regulation of blood microflow in vivo represents a significant method for investigating illnesses linked to abnormal changes in blood circulation. Currently, non-invasive strategies are limited to regulation within capillaries of approximately 10 μm in diameter because the adaption to blood pressure levels in the order of several hundred pascals poses a significant challenge in larger microvessels. In this study, using laser-induced microbubble formation within microvessels of the mouse auricle, we regulate blood microflow in small vessels with diameters in the tens of micrometers. By controlling the laser power, we can control the growth and stability of microbubbles in vivo. This controlled approach enables the achievement of prolonged ischemia and subsequent reperfusion of blood flow, and it can also regulate the microbubbles to function as micro-pumps for reverse blood pumping. Furthermore, by controlling the microbubble, narrow microflow channels can be formed between the microbubbles and microvessels for assessing the apparent viscosity of leukocytes, which is 76.9 ± 11.8 Pa·s in the in vivo blood environment. The proposed design of in vivo microbubble valves opens new avenues for constructing real-time blood regulation and exploring cellular mechanics within living organisms.
中文翻译：
激光诱导微泡作为体内阀门，用于活体小鼠微血管中的光流控操作
体内血液微流的光流控调节是研究与血液循环异常变化相关的疾病的重要方法。目前，非侵入性策略仅限于直径约 10 μm 的毛细血管内的调节，因为适应数百帕斯卡的血压水平对较大的微血管构成了重大挑战。在这项研究中，我们利用激光诱导小鼠耳廓微血管内的微泡形成，调节直径数十微米的小血管中的血液微流量。通过控制激光功率，我们可以控制体内微泡的生长和稳定性。这种受控方法能够实现长时间的缺血和随后的血流再灌注，并且还可以调节微泡以充当反向血液泵送的微型泵。此外，通过控制微泡，可以在微泡和微血管之间形成狭窄的微流道，用于评估白细胞的表观粘度，在体内血液环境中，表观粘度为76.9±11.8Pa·s。所提出的体内微泡阀设计为构建实时血液调节和探索生物体内的细胞力学开辟了新途径。
更新日期：2024-06-13
中文翻译：
激光诱导微泡作为体内阀门，用于活体小鼠微血管中的光流控操作
体内血液微流的光流控调节是研究与血液循环异常变化相关的疾病的重要方法。目前，非侵入性策略仅限于直径约 10 μm 的毛细血管内的调节，因为适应数百帕斯卡的血压水平对较大的微血管构成了重大挑战。在这项研究中，我们利用激光诱导小鼠耳廓微血管内的微泡形成，调节直径数十微米的小血管中的血液微流量。通过控制激光功率，我们可以控制体内微泡的生长和稳定性。这种受控方法能够实现长时间的缺血和随后的血流再灌注，并且还可以调节微泡以充当反向血液泵送的微型泵。此外，通过控制微泡，可以在微泡和微血管之间形成狭窄的微流道，用于评估白细胞的表观粘度，在体内血液环境中，表观粘度为76.9±11.8Pa·s。所提出的体内微泡阀设计为构建实时血液调节和探索生物体内的细胞力学开辟了新途径。
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