Isolation and characterization of a phage collection against Pseudomonas putida,Environmental Microbiology

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Isolation and characterization of a phage collection against Pseudomonas putida
Environmental Microbiology ( IF 4.3 ) Pub Date : 2024-06-11 , DOI: 10.1111/1462-2920.16671
Age Brauer ₁ , Sirli Rosendahl ₂ , Anu Kängsep ₂ , Alicja Cecylia Lewańczyk ₂ , Roger Rikberg ₂ , Rita Hõrak ₂ , Hedvig Tamman ₂

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The environmental bacterium, Pseudomonas putida, possesses a broad spectrum of metabolic pathways. This makes it highly promising for use in biotechnological production as a cell factory, as well as in bioremediation strategies to degrade various aromatic pollutants. For P. putida to flourish in its environment, it must withstand the continuous threats posed by bacteriophages. Interestingly, until now, only a handful of phages have been isolated for the commonly used laboratory strain, P. putida KT2440, and no phage defence mechanisms have been characterized. In this study, we present a new Collection of Environmental P. putida Phages from Estonia, or CEPEST. This collection comprises 67 double-stranded DNA phages, which belong to 22 phage species and 9 phage genera. Our findings reveal that most phages in the CEPEST collection are more infectious at lower temperatures, have a narrow host range, and require an intact lipopolysaccharide for P. putida infection. Furthermore, we show that cryptic prophages present in the P. putida chromosome provide strong protection against the infection of many phages. However, the chromosomal toxin–antitoxin systems do not play a role in the phage defence of P. putida. This research provides valuable insights into the interactions between P. putida and bacteriophages, which could have significant implications for biotechnological and environmental applications.

中文翻译：

针对恶臭假单胞菌的噬菌体集合的分离和表征

环境细菌恶臭假单胞菌具有广泛的代谢途径。这使得它非常有希望作为细胞工厂用于生物技术生产，以及用于降解各种芳香污染物的生物修复策略。为了使恶臭假单胞菌在其环境中繁衍生息，它必须承受噬菌体带来的持续威胁。有趣的是，到目前为止，仅分离出少数常用实验室菌株恶臭假单胞菌KT2440 的噬菌体，并且尚未表征噬菌体防御机制。在这项研究中，我们展示了来自爱沙尼亚的新环境恶臭假单胞菌噬菌体集合（CEPEST）。该集合包含 67 个双链 DNA 噬菌体，属于 22 个噬菌体种和 9 个噬菌体属。我们的研究结果表明，CEPEST 收集中的大多数噬菌体在较低温度下更具传染性，宿主范围较窄，并且需要完整的脂多糖才能感染恶臭假单胞菌。此外，我们发现恶臭假单胞菌染色体中存在的隐性前噬菌体可以提供强有力的保护，防止许多噬菌体的感染。然而，染色体毒素-抗毒素系统在恶臭假单胞菌的噬菌体防御中不起作用。这项研究为恶臭假单胞菌和噬菌体之间的相互作用提供了宝贵的见解，这可能对生物技术和环境应用产生重大影响。

更新日期：2024-06-12

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