Two Cytochrome P450s, CYP709B1 and CYP704C1, Play Essential Roles in Metabolism-Based Multiple Herbicide Resistance in American Sloughgrass (Beckmannia syzigachne (Steud.) Fernald),Journal of Agricultural and Food Chemistry

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Two Cytochrome P450s, CYP709B1 and CYP704C1, Play Essential Roles in Metabolism-Based Multiple Herbicide Resistance in American Sloughgrass (Beckmannia syzigachne (Steud.) Fernald)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-06-11 , DOI: 10.1021/acs.jafc.4c02674
Fan Yin _{1,

2,

3,

4} , Zilu Wang _{1,

2,

3,

4} , Minghao Jiang _{1,

2,

3,

4} , Min Liao _{1,

2,

3,

4} , Haiqun Cao _{1,

2,

3,

4} , Ning Zhao _{1,

2,

3,

4}

Affiliation

This study confirmed a field population of American sloughgrass (Beckmannia syzigachne (Steud.) Fernald) that developed simultaneously high levels of resistance (resistance index >10) to three divergent modes of action herbicides: fenoxaprop-P-ethyl, mesosulfuron-methyl, and isoproturon. The resistance phenotype observed in this population was not attributed to target-site alterations; rather, the resistant plants exhibited a significant increase in the activity of cytochrome P450s (P450s) and enhanced metabolism rates for all three herbicides. RNA sequencing revealed significant upregulation of two P450s, CYP709B1 and CYP704C1, in the resistant plants both before and after herbicide treatments. Molecular docking predicted that the homology models of these P450s should exhibit a binding affinity for a range of herbicides. The heterologous expression of the identified P450s in yeast cells indicated improved growth in the presence of all three of the aforementioned herbicides. Collectively, the increased expression of CYP709B1 and CYP704C1 likely contributed to the P450s-mediated enhanced metabolism, thereby conferring multiple herbicide resistance in B. syzigachne.

中文翻译：

两种细胞色素 P450，CYP709B1 和 CYP704C1，在美国沼泽草（Beckmannia syzigachne （Steud.）Fernald）

这项研究证实了美国沼泽草（Beckmannia syzigachne （Steud.）Fernald）同时对三种不同作用模式的除草剂产生了高水平的抗性（抗性指数 >10）：fenoxaprop-P-ethyl、mesosulfuron-methyl 和 isoproturon。在该人群中观察到的耐药表型不归因于靶位点改变;相反，抗性植物表现出细胞色素 P450 （P450）活性的显着增加和所有三种除草剂的新陈代谢速率的增强。RNA 测序显示，在除草剂处理前后，抗性植物中两种 P450 （CYP709B1 和 CYP704C1）显著上调。分子对接预测这些 P450 的同源模型应该表现出对一系列除草剂的结合亲和力。鉴定的 P450 在酵母细胞中的异源表达表明，在上述所有三种除草剂存在下生长改善。总的来说，CYP709B1 和 CYP704C1 表达的增加可能有助于 P450s 介导的代谢增强，从而赋予 B. syzigachne 多种除草剂抗性。

更新日期：2024-06-11

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