Time-programmed release of curcumin and Zn2+ from multi-layered RSF coating modified PET graft for improvement of graft-host integration,International Journal of Biological Macromolecules

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Time-programmed release of curcumin and Zn2+ from multi-layered RSF coating modified PET graft for improvement of graft-host integration
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2024-05-31 , DOI: 10.1016/j.ijbiomac.2024.132830
Han Gao ₁ , Ni Chen ₂ , Luyi Sun ₁ , Dandan Sheng ₁ , Yuting Zhong ₁ , Mingru Huang ₁ , Chengxuan Yu ₁ , Xing Yang ₃ , Yuefeng Hao ₃ , Shiyi Chen ₁ , Zhengzhong Shao ₂ , Jun Chen ₁

Affiliation

Artificial graft serves as the primary grafts used in the clinical management of sports-related injuries. Until now, optimizing its graft-host integration remains a great challenge due to the excessive inflammatory response during the inflammatory phase, coupled with an absence of tissue-inductive capacity during the regeneration phase. Here, a multi-layered regenerated silk fibroin (RSF) coating loaded with curcumin (Cur) and Zn on the surface of the PET grafts (Cur@Zn@PET) was designed and fabricated for providing time-matched regulation specifically tailored to address issues arising at both inflammatory and regeneration phases, respectively. The release of Cur and Zn from the Cur@Zn@PET followed a time-programmed pattern in vitro. Specifically, cellular assays revealed that Cur@Zn@PET initially released Cur during the inflammatory phase, thereby markedly inhibit the expression of inflammatory cytokines TNF-a and IL-1β. Meanwhile, a significant release of Zn was major part during the regeneration phase, serving to induce the osteogenic differentiation of rBMSC. Furthermore, rat model of anterior cruciate ligament reconstruction (ACLR) showed that through time-programmed drug release, Cur@Zn@PET could suppress the formation of fibrous interface (FI) caused by inflammatory response, combined with significant new bone (NB) formation during regeneration phase. Consequently, the implementation of the Cur@Zn@PET characterized by its time-programmed release patterns hold considerable promise for improving graft-host integration for sports-related injuries.

中文翻译：

从多层 RSF 涂层改性 PET 接枝物中定时释放姜黄素和 Zn2+，以改善接枝物与宿主的整合

人工移植物是运动相关损伤临床治疗中使用的主要移植物。到目前为止，由于炎症阶段的过度炎症反应以及再生阶段缺乏组织诱导能力，优化其移植物-宿主整合仍然是一个巨大的挑战。这里，设计并制造了在 PET 接枝物 (Cur@Zn@PET) 表面负载姜黄素 (Cur) 和 Zn 的多层再生丝素蛋白 (RSF) 涂层，以提供专门针对解决问题而设计的时间匹配调节分别出现在炎症和再生阶段。在体外，Cur@Zn@PET 中 Cur 和 Zn 的释放遵循时间编程模式。具体来说，细胞分析表明，Cur@Zn@PET 在炎症阶段最初释放 Cur，从而显着抑制炎症细胞因子 TNF-a 和 IL-1β 的表达。同时，Zn的显着释放是再生阶段的主要部分，有助于诱导rBMSC的成骨分化。此外，大鼠前交叉韧带重建（ACLR）模型表明，通过时间程序药物释放，Cur@Zn@PET可以抑制炎症反应引起的纤维界面（FI）的形成，并伴有显着的新骨（NB）形成在再生阶段。因此，以时间编程释放模式为特征的 Cur@Zn@PET 的实施对于改善运动相关损伤的移植物-宿主整合具有很大的希望。

更新日期：2024-05-31

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