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Maintain the light, long-term seasonal monitoring of luminous capabilities in the brittle star Amphiura filiformis
Scientific Reports ( IF 3.8 ) Pub Date : 2024-06-09 , DOI: 10.1038/s41598-024-64010-x
Constance Coubris 1 , Laurent Duchatelet 1 , Jérôme Delroisse 2, 3 , Wendy Shirley Bayaert 2 , Laura Parise 1 , Marie Christine Eloy 4 , Christophe Pels 1 , Jérôme Mallefet 1
Affiliation  

The European brittle star Amphiura filiformis emits blue light, via a Renilla-like luciferase, which depends on the dietary acquisition of coelenterazine. Questions remain regarding luciferin availability across seasons and the persistence of luminous capabilities after a single boost of coelenterazine. To date, no study has explored the seasonal, long-term monitoring of these luminous capabilities or the tracking of luciferase expression in photogenic tissues. Through multidisciplinary analysis, we demonstrate that luminous capabilities evolve according to the exogenous acquisition of coelenterazine throughout adult life. Moreover, no coelenterazine storage forms are detected within the arms tissues. Luciferase expression persists throughout the seasons, and coelenterazine's presence in the brittle star diet is the only limiting factor for the bioluminescent reaction. No seasonal variation is observed, involving a continuous presence of prey containing coelenterazine. The ultrastructure description provides a morphological context to investigate the green autofluorescence signal attributed to coelenterazine during luciferin acquisition. Finally, histological analyses support the hypothesis of a pigmented sheath leading light to the tip of the spine. These insights improve our understanding of the bioluminescence phenomenon in this burrowing brittle star.



中文翻译:


对海蛇尾的发光能力进行长期的季节性监测



欧洲海蛇尾Amphiura filiformis通过类似海肾的荧光素酶发出蓝光,这取决于从饮食中获取腔肠素。关于荧光素跨季节的可用性以及腔肠素单次增强后发光能力的持久性仍然存在问题。迄今为止,还没有研究探索对这些发光能力的季节性、长期监测或对上镜组织中荧光素酶表达的跟踪。通过多学科分析,我们证明发光能力是根据整个成年期腔肠素的外源获取而进化的。此外,在手臂组织内没有检测到腔肠素储存形式。荧光素酶的表达在整个季节中持续存在,而海星饮食中腔肠素的存在是生物发光反应的唯一限制因素。没有观察到季节性变化,涉及含有腔肠素的猎物的持续存在。超微结构描述提供了形态学背景来研究荧光素采集过程中腔肠素引起的绿色自发荧光信号。最后,组织学分析支持了色素鞘将光线引导至脊柱尖端的假设。这些见解提高了我们对这种穴居海蛇尾的生物发光现象的理解。

更新日期:2024-06-09
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