<br>转录组分析揭示不同发育阶段绵羊肌肉组织基因表达的差异,BMC Genetics

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转录组分析揭示不同发育阶段绵羊肌肉组织基因表达的差异
BMC Genetics Pub Date : 2024-06-07 , DOI: 10.1186/s12863-024-01235-9
Sailuo Wan _{1,

2} , Mengyu Lou _{2,

3} , Sihuan Zhang _{2,

3} , Shuang Li _{2,

3} , Yinghui Ling _{2,

3}

Affiliation

分析不同年龄段绵羊肌肉组织中差异表达基因，有助于分析肌肉发育过程中基因表达趋势。本研究中纯种湖羊（H）、萨福克羊与湖羊杂交F1代（SH）以及东弗里斯兰与湖羊杂交羊（EHH）3个品系羊出生2天（B2）的背最长肌以8月龄（M8）为研究对象，利用转录组测序技术鉴定羊背最长肌在这两个阶段的差异表达基因。随后对差异基因进行GO和KEGG富集分析。随机选择9个差异表达基因，通过qRT-PCR验证其表达水平。结果显示，H组、SH组和EHH组分别鉴定出842个、1301个和1137个差异表达基因。其中，这3个菌株富集了191个差异基因，包括预折叠蛋白亚基6（PFDN6）、DnaJ热休克蛋白家族成员A4（DNAJA4）、肌球蛋白重链8（MYH8）等。对三个菌株共有的191个差异表达基因进行GO和KEGG富集分析，以确定共同的生物学途径。结果显示，差异表达基因显着富集于调节肌肉蛋白合成和能量代谢的核糖体、未折叠蛋白结合、FoxO信号通路、糖酵解/糖原生成和谷胱甘肽信号通路。 qRT-PCR结果与转录组测序结果一致，证明测序结果可靠。总体而言，本研究揭示了与绵羊骨骼肌发育相关的重要基因和信号通路，为进一步了解绵羊骨骼肌发育机制奠定了基础。

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Transcriptome analysis revealed differences in gene expression in sheep muscle tissue at different developmental stages

The analysis of differentially expressed genes in muscle tissues of sheep at different ages is helpful to analyze the gene expression trends during muscle development. In this study, the longissimus dorsi muscle of pure breeding Hu sheep (H), Suffolk sheep and Hu sheep hybrid F1 generation (SH) and East Friesian and Hu sheep hybrid sheep (EHH) three strains of sheep born 2 days (B2) and 8 months (M8) was used as the research object, and transcriptome sequencing technology was used to identify the differentially expressed genes of sheep longissimus dorsi muscle in these two stages. Subsequently, GO and KEGG enrichment analysis were performed on the differential genes. Nine differentially expressed genes were randomly selected and their expression levels were verified by qRT-PCR. The results showed that 842, 1301 and 1137 differentially expressed genes were identified in H group, SH group and EHH group, respectively. Among them, 191 differential genes were enriched in these three strains, including pre-folding protein subunit 6 (PFDN6), DnaJ heat shock protein family member A4 (DNAJA4), myosin heavy chain 8 (MYH8) and so on. GO and KEGG enrichment analysis was performed on 191 differentially expressed genes shared by the three strains to determine common biological pathways. The results showed that the differentially expressed genes were significantly enriched in ribosomes, unfolded protein binding, FoxO signaling pathway, glycolysis / glycogen generation and glutathione signaling pathway that regulate muscle protein synthesis and energy metabolism. The results of qRT-PCR were consistent with transcriptome sequencing, which proved that the sequencing results were reliable. Overall, this study revealed the important genes and signaling pathways related to sheep skeletal muscle development, and the result laid a foundation for further understanding the mechanism of sheep skeletal muscle development.

更新日期：2024-06-08

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