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Highly Branched Rhamnogalacturonan-I Type Pectin from Wolfberry: Purification, Characterization, and Structure-Prebiotic/Immunomodulatory Activity Relationship
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-06-06 , DOI: 10.1021/acs.jafc.4c03140
Jinyan Gu 1, 2 , Lianzhu Lin 1, 2 , Mouming Zhao 1, 2
Affiliation  

Due to the difficulty in obtaining highly branched rhamnogalacturonan-I (RG-I) type pectin, the relationship between the extent of RG-I branching (EB) of pectin and prebiotic/immunomodulatory activity has not been systematically investigated. Moreover, it is only possible to establish a structure–activity relationship using pectin that is highly purified and accurately characterized. In this study, a homogeneous highly branched RG-I type pectin (LBP-P4, a final product) with dual proliferative effects on Bifidobacterium and macrophage was effectively purified for the first time using enzyme hydrolysis combined with ultrafiltration. The RG-I content and EB of LBP-P4 reached 97.32 and 77.12, respectively. Its two branches were composed of arabinan and arabinogalactan-II, containing → 5)-Araf-(1→, →3)-Araf-(1→, →3,6)-Galp-(1→ and →6)-Galp-(1→ residues). The structure–activity relationship analysis indicated that strong prebiotic/immunomodulatory activity of LBP-P4 was depended on its high EB, which was further confirmed by the molecular docking simulation between low/high branched pectin with β-1,6-galactosidase, α-l-arabinanase, and Toll-like receptor 4 (TLR4).

中文翻译:


枸杞中的高度支化鼠李糖半乳糖醛酸-I 型果胶:纯化、表征和结构-益生元/免疫调节活性关系



由于难以获得高度支化的鼠李糖半乳糖醛酸-I (RG-I)型果胶,果胶的RG-I支化程度(EB)与益生元/免疫调节活性之间的关系尚未得到系统研究。此外,只有使用高度纯化和准确表征的果胶才能建立结构-活性关系。本研究首次采用酶水解结合超滤的方法,有效纯化了一种对双歧杆菌和巨噬细胞具有双重增殖作用的均质高支化RG-I型果胶(LBP-P4,最终产品)。 LBP-P4的RG-I含量和EB分别达到97.32和77.12。其两个分支由阿拉伯聚糖和阿拉伯半乳聚糖-II组成,含有→5)-Araf-(1→,→3)-Araf-(1→,→3,6)-Galp-(1→和→6)-Galp -(1→残基)。构效关系分析表明,LBP-P4的强益生元/免疫调节活性取决于其高EB,这通过低/高支化果胶与β-1,6-半乳糖苷酶、α- L-阿拉伯聚糖酶和 Toll 样受体 4 (TLR4)。
更新日期:2024-06-06
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