Using next-generation cryo-EM and mass spectrometry, we identified 235 chemical modifications in the sub-2 Å resolution structure of the full human 80S ribosome. The newly identified rRNA modifications were found to create new hydrogen bond patterns for riboses and uridines. Ion visualization revealed that Mg2+-associated water molecules are variably substituted by side chains. This study provides the molecular basis for the stabilization of A–U or A–Ψ base pairs and RNA–protein interactions.

中文翻译：

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人类 80S 核糖体亚 2 Å 分辨率结构中的化学修饰、离子和水分子


使用下一代冷冻电镜和质谱分析，我们在完整的人类 80S 核糖体的亚 2 Å 分辨率结构中鉴定出了 235 个化学修饰。新发现的 rRNA 修饰可以为核糖和尿苷创造新的氢键模式。离子可视化显示 Mg2+ 相关水分子不同程度地被侧链取代。这项研究为 A-U 或 A-Ψ 碱基对和 RNA-蛋白质相互作用的稳定提供了分子基础。