Injectable, tissue mimetic, bioactive, and biodegradable hydrogels offer less invasive regeneration and repair of tissues. The monitoring swelling and in vitro degradation capacities of hydrogels are highly important for drug delivery and tissue regeneration processes. Bioactivity of bone tissue engineered constructs in terms of mineralized apatite formation capacity is also pivotal. We have previously reported in situ forming chitosan-based injectable hydrogels integrated with hydroxyapatite and heparin for bone regeneration, promoting angiogenesis. These hydrogels were functionalized by glycerol and pH to improve their mechano-structural properties. In the present study, functionalized hybrid hydrogels were investigated for their swelling, in vitro degradation, and bioactivity performances. Hydrogels have degraded gradually in phosphate-buffered saline (PBS) with and without lysozyme enzyme. The percentage weight loss of hydrogels and their morphological and chemical properties, and pH of media were analyzed. The swelling ratio of hydrogels (55%–68%(wt), 6 h of equilibrium) indicated a high degree of cross-linking, can be suitable for controlled drug release. Hydrogels have gradually degraded reaching to 60%–70% (wt%) in 42 days in the presence and absence of lysozyme, respectively. Simulated body fluid (SBF)-treated hydrogels containing hydroxyapatite-induced needle-like carbonated-apatite mineralization was further enhanced by heparin content significantly.

中文翻译：

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用于骨再生和药物输送的原位形成可注射壳聚糖基质水凝胶的体外降解、膨胀和生物活性性能


可注射、仿组织、生物活性和可生物降解的水凝胶提供侵入性较小的组织再生和修复。监测水凝胶的膨胀和体外降解能力对于药物输送和组织再生过程非常重要。骨组织工程构建体在矿化磷灰石形成能力方面的生物活性也至关重要。我们之前曾报道过原位形成基于壳聚糖的可注射水凝胶与羟基磷灰石和肝素的结合，用于骨再生，促进血管生成。这些水凝胶通过甘油和 pH 值进行功能化，以改善其机械结构特性。在本研究中，研究了功能化杂化水凝胶的膨胀、体外降解和生物活性性能。水凝胶在有或没有溶菌酶的磷酸盐缓冲盐水 (PBS) 中逐渐降解。分析了水凝胶的重量损失百分比及其形态和化学性质以及介质的 pH 值。水凝胶的溶胀率（55%–68%（wt），平衡6小时）表明交联程度高，适合药物控制释放。在存在溶菌酶和不存在溶菌酶的情况下，水凝胶在 42 天内逐渐降解至 60%–70% (wt%)。经模拟体液（SBF）处理的含有羟基磷灰石的水凝胶诱导的针状碳酸磷灰石矿化进一步因肝素含量而显着增强。