Fluorescence microscopy: A statistics-optics perspective,Reviews of Modern Physics

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Fluorescence microscopy: A statistics-optics perspective
Reviews of Modern Physics ( IF 45.9 ) Pub Date : 2024-06-05 , DOI: 10.1103/revmodphys.96.025003
Mohamadreza Fazel _{1,

1} , Kristin S. Grussmayer ₂ , Boris Ferdman ₃ , Aleksandra Radenovic ₄ , Yoav Shechtman ₃ , Jörg Enderlein ₅ , Steve Pressé _{1,

1,

1}

Affiliation

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scales on par with or smaller than light’s emission wavelength. Here the optics responsible for generating fluorescent images, fluorophore properties, and microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement, are reviewed.

中文翻译：

荧光显微镜：统计学-光学视角

光的基本特性不可避免地会对使用荧光显微镜采集的图像产生影响。在定量解释显微镜图像时，考虑这些特征通常至关重要，尤其是那些以与光发射波长相当或更小的尺度收集信息的图像。这里回顾了负责生成荧光图像的光学器件、荧光团特性和利用光和荧光团特性的显微镜模式，以及由光和测量的随机性质施加的必要概率建模工具。

更新日期：2024-06-05

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