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High-fidelity and fast 3D imaging of subcellular dynamics in native states
Nature Biotechnology ( IF 33.1 ) Pub Date : 2024-06-04 , DOI: 10.1038/s41587-024-02293-1


By integrating confocal illumination along a moving axis with scanning light-field microscopy, confocal scanning light-field microscopy captures images with high spatiotemporal resolution and optical sectioning simultaneously, with low phototoxicity. This compact technique enabled us to investigate a wide variety of rapid subcellular 3D dynamics in vivo with high fidelity over extended periods.

中文翻译:


天然状态下亚细胞动力学的高保真快速 3D 成像



通过将沿移动轴的共焦照明与扫描光场显微镜相结合,共焦扫描光场显微镜可以同时捕获具有高时空分辨率和光学切片的图像,并且光毒性较低。这种紧凑的技术使我们能够在长时间内以高保真度研究各种快速的体内亚细胞 3D 动力学。
更新日期:2024-06-04
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