Fruit ripening, which is modulated by the up- and downregulation of numerous genes, is a sophisticated physiological event determining consumer acceptability. While many positive regulators have been known to regulate fruit ripening, relatively less information is associated with the negative regulators in the process. Here, a negative regulator, , was characterized according to the banana fruit ripening transcriptome, which displayed nuclear localization and inhibitory transactivation activity. MaMADS31 suppresses the transcription of the cell wall modification gene and the ethylene biosynthesis-related gene 3 by directly recognizing the CArG-box element in their promoters. Transient expression of in banana fruit brought about downregulation of and , thereby delaying fruit ripening. Importantly, MaMADS31 interacts with MaBZR2 to synergistically strengthen the transcriptional inhibition of and . Overall, MaMADS31-MaBZR2 plays a negative role in fruit ripening by downregulating the and transcription, which provides new insights for innovating approaches for prolonging the postharvest life of horticultural plants.

中文翻译：

---

MaMADS31-MaBZR2 的新型模块对香蕉果实成熟具有负调控作用


果实成熟是由众多基因的上调和下调调节的，是决定消费者可接受性的复杂生理事件。虽然已知许多正调节因子可以调节果实成熟，但与该过程中负调节因子相关的信息相对较少。在此，根据香蕉果实成熟转录组对负调节因子 进行了表征，该转录组显示出核定位和抑制反式激活活性。 MaMADS31通过直接识别启动子中的CArG-box元件来抑制细胞壁修饰基因和乙烯生物合成相关基因3的转录。香蕉果实中的瞬时表达导致 和 的下调，从而延迟果实成熟。重要的是，MaMADS31 与 MaBZR2 相互作用，协同加强 和 的转录抑制。总体而言，MaMADS31-MaBZR2 通过下调 和 转录在果实成熟中发挥负面作用，这为延长园艺植物采后寿命的创新方法提供了新的见解。