Engineering Ribosomal Machinery for Noncanonical Amino Acid Incorporation,Chemical Reviews

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Engineering Ribosomal Machinery for Noncanonical Amino Acid Incorporation
Chemical Reviews ( IF 51.4 ) Pub Date : 2024-06-03 , DOI: 10.1021/acs.chemrev.3c00912
Satoshi Ishida ₁ , Phuoc H T Ngo ₁ , Arno Gundlach ₁ , Andrew Ellington ₁

Affiliation

The introduction of noncanonical amino acids into proteins has enabled researchers to modify fundamental physicochemical and functional properties of proteins. While the alteration of the genetic code, via the introduction of orthogonal aminoacyl-tRNA synthetase:tRNA pairs, has driven many of these efforts, the various components involved in the process of translation are important for the development of new genetic codes. In this review, we will focus on recent advances in engineering ribosomal machinery for noncanonical amino acid incorporation and genetic code modification. The engineering of the ribosome itself will be considered, as well as the many factors that interact closely with the ribosome, including both tRNAs and accessory factors, such as the all-important EF-Tu. Given the success of genome re-engineering efforts, future paths for radical alterations of the genetic code will require more expansive alterations in the translation machinery.

中文翻译：

用于非规范氨基酸掺入的核糖体机械工程

将非规范氨基酸引入蛋白质中使研究人员能够修改蛋白质的基本理化和功能特性。虽然通过引入正交氨酰基-tRNA合成酶：tRNA对而改变遗传密码推动了许多此类努力，但翻译过程中涉及的各种成分对于新遗传密码的开发也很重要。在这篇综述中，我们将重点关注用于非规范氨基酸掺入和遗传密码修饰的核糖体机械工程的最新进展。将考虑核糖体本身的工程，以及与核糖体密切相互作用的许多因素，包括 tRNA 和辅助因素，例如最重要的 EF-Tu。鉴于基因组重新工程工作的成功，未来彻底改变遗传密码的途径将需要对翻译机制进行更广泛的改变。

更新日期：2024-06-03

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