Optically active alcohols are crucial precursors. 1,2-Diarylethanols and their analogues constitute a significant group of substances of biological significance. The biocatalytic synthesis of ()-1,2-diphenylethanol (), which can be utilized as a precursor to important drugs, is still not at the desired level in terms of substrate amount and production process. This study asymmetrically reduced 1,2-diphenylethanone using the BD87E6 biocatalyst and a novel orthogonal quadratic design-embedded optimization approach. The following reaction optimization conditions were determined using the suggested optimization technique: pH = 6, temperature = 31 °C, incubation period = 48 h, and agitation speed=134 rpm. In addition, the reaction conversion was estimated to be 99.38 %, and the product's enantiomeric excess (ee) was estimated to be 99.12 %. Further, was produced with >99 % ee, >99 % conversion, and 98 % yield in the experimental investigation under the established optimum conditions. This study is the first attempt to reduce substrate to or using a mathematical optimization method in the presence of a biocatalyst. Furthermore, on a high-gram scale, (15.70 g) was entirely transformed into (15.54 g, 98 % isolated yield). Notably, this study is also the first to perform the gram-scale production of using a novel optimization approach and a biocatalyst. Finally, the innovative and novel orthogonal quadratic design-embedded optimization technique has been demonstrated as an efficient, cost-effective, and environmentally friendly biocatalytic procedure for synthesizing .

中文翻译：

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使用全细胞生物催化剂有效生物催化合成对映体纯 (R)-1,2-二苯乙醇作为药物前体


光学活性醇是重要的前体。 1,2-二芳基乙醇及其类似物构成了一组具有生物学意义的重要物质。 ()-1,2-二苯乙醇()可作为重要药物的前体，其生物催化合成在底物量和生产工艺方面仍未达到理想水平。本研究使用 BD87E6 生物催化剂和新颖的正交二次设计嵌入式优化方法不对称还原 1,2-二苯乙酮。使用建议的优化技术确定以下反应优化条件：pH = 6，温度 = 31 °C，孵育时间 = 48 小时，搅拌速度 = 134 rpm。此外，反应转化率估计为99.38％，产物的对映体过量(ee)估计为99.12％。此外，在已确定的最佳条件下进行的实验研究中，生成的产物具有 >99% ee、>99% 的转化率和 98% 的产率。这项研究是在生物催化剂存在下减少底物或使用数学优化方法的首次尝试。此外，在高克规模上，(15.70g)完全转化为(15.54g，98%分离产率)。值得注意的是，这项研究也是第一个使用新型优化方法和生物催化剂进行克级生产的研究。最后，创新的正交二次设计嵌入式优化技术已被证明是一种高效、经济且环保的生物催化合成方法。