The de novo synthesis of genomes has made unprecedented progress and achieved milestones, particularly in bacteria and yeast. However, the process of synthesizing a multicellular plant genome has not progressed at the same pace, due to the complexity of multicellular plant genomes, technical difficulties associated with large genome size and structure, and the intricacies of gene regulation and expression in plants. Here we outline the bottom-up design principles for the de novo synthesis of the Physcomitrium patens (that is, earthmoss) genome. To facilitate international collaboration and accessibility, we have developed and launched a public online design platform called GenoDesigner. This platform offers an intuitive graphical interface enabling users to efficiently manipulate extensive genome sequences, even up to the gigabase level. This tool is poised to greatly expedite the synthesis of the P. patens genome, offering an essential reference and roadmap for the synthesis of plant genomes.

基因组的从头合成取得了前所未有的进展并实现了里程碑，特别是在细菌和酵母中。然而，由于多细胞植物基因组的复杂性、与大基因组大小和结构相关的技术困难以及植物中基因调控和表达的复杂性，合成多细胞植物基因组的过程并没有以同样的速度进展。在这里，我们概述了小立碗藓（即土苔）基因组从头合成的自下而上的设计原则。为了促进国际合作和可及性，我们开发并推出了一个名为 GenoDesigner 的公共在线设计平台。该平台提供直观的图形界面，使用户能够有效地操作广泛的基因组序列，甚至高达千兆碱基水平。该工具有望大大加快P. patens基因组的合成，为植物基因组的合成提供重要的参考和路线图。