Hand-held all-in-one (HAO) self-test kit for rapid and on-site detection of SARS-CoV-2 with colorimetric LAMP,Lab on a Chip

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Hand-held all-in-one (HAO) self-test kit for rapid and on-site detection of SARS-CoV-2 with colorimetric LAMP
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-06-03 , DOI: 10.1039/d4lc00199k
Qingyang Wang ₁ , Woong Heo ₁ , Seoyeon Choi _{1,

2} , Woongsik Jang ₃ , Chae Seung Lim ₃ , Hyo-Il Jung _{1,

2}

Affiliation

Throughout the COVID-19 pandemic, individuals potentially infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were forcibly recalled to local or central hospitals, where the diagnostic results were obtained a couple of days after the liquid biopsies were subjected to conventional polymerase chain reaction (PCR). This slow output of such a complex and time-consuming laboratory procedure hindered its widespread application. To overcome the limitations associated with such a centralized diagnostic system, we developed a hand-held and all-in-one type test kit in which the analytical results can be obtained in only 30 min. The test kit consists of three major steps for on-site SARS-CoV-2 RNA detection: 1) virus lysis by heat, 2) RNA enrichment by membrane, and 3) real-time detection by colorimetric loop-mediated isothermal amplification (c-LAMP). The proposed device operates in a sample-to-answer format, is fully automated, and reduces dependence on traditional laboratory settings, facilitating large-scale population screening.

中文翻译：

手持式一体式 (HAO) 自检套件，可使用比色 LAMP 快速现场检测 SARS-CoV-2

在整个 COVID-19 大流行期间，可能感染严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的个人被强行召回当地或中心医院，在进行液体活检几天后获得诊断结果常规聚合酶链反应（PCR）。这种复杂且耗时的实验室程序的缓慢产出阻碍了其广泛应用。为了克服这种集中诊断系统的局限性，我们开发了一种手持式一体化测试套件，只需 30 分钟即可获得分析结果。该检测试剂盒包括现场 SARS-CoV-2 RNA 检测的三个主要步骤：1) 热裂解病毒，2) 通过膜富集 RNA，3) 通过比色环介导的等温扩增（c）进行实时检测。 -灯）。所提出的设备以样本到答案的形式运行，完全自动化，并减少对传统实验室设置的依赖，促进大规模人群筛查。

更新日期：2024-06-07

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