<br>综合代谢组学、酶活性和基因表达分析提供了对伪鲛牙慢肌和快肌之间代谢谱差异的见解,International Journal of Molecular Sciences

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综合代谢组学、酶活性和基因表达分析提供了对伪鲛牙慢肌和快肌之间代谢谱差异的见解
International Journal of Molecular Sciences ( IF 4.9 ) Pub Date : 2024-06-01 , DOI: 10.3390/ijms25116131
Huan Wang ₁ , Busu Li _{1,

2} , Ang Li ₁ , Changting An ₁ , Shufang Liu _{1,

2} , Zhimeng Zhuang ₁

Affiliation

硬骨鱼的骨骼肌包含异质肌肉类型，称为慢肌（SM）和快肌（FM），其特征在于不同的形态、解剖、组织学、生化和生理属性，驱动不同的游泳行为。尽管代谢在调节骨骼肌类型和功能中发挥着核心作用，但缺乏针对这些肌肉类型之间代谢差异的全面代谢组学研究。为了揭示硬骨鱼SM和FM代谢特征的差异，我们以Pseudocaranx dentex为代表模型进行了非靶向代谢组学分析，鉴定了411种差异代谢物（DFM），其中345种在SM中含量较高，66种在FM中含量较高。 KEGG富集分析表明，这些DFM在脂质、氨基酸、碳水化合物、嘌呤和维生素的代谢过程中富集，表明SM和FM在多个代谢途径中存在显着差异，特别是在能量物质的代谢方面。此外，对ATP-PCr磷酸、无氧糖酵解和有氧氧化能量系统涉及的代谢物含量、酶活性测定和基因表达水平进行综合分析，以探索能量代谢差异的潜在调节机制。结果揭示了 SM 和 FM 之间的一组差异代谢物、酶和基因，提供了令人信服的分子证据，证明 FM 通过 ATP-PCr 磷酸盐和糖酵解能量系统实现了更高的无氧供能能力，而 SM 获得了更大的能量通过有氧氧化的供应能力。这些发现极大地促进了我们对骨骼肌代谢特征和相关调节机制的理解，从而扩大了硬骨鱼代谢生理学和生态适应的知识。

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Integrative Metabolomics, Enzymatic Activity, and Gene Expression Analysis Provide Insights into the Metabolic Profile Differences between the Slow-Twitch Muscle and Fast-Twitch Muscle of Pseudocaranx dentex

The skeletal muscles of teleost fish encompass heterogeneous muscle types, termed slow-twitch muscle (SM) and fast-twitch muscle (FM), characterized by distinct morphological, anatomical, histological, biochemical, and physiological attributes, driving different swimming behaviors. Despite the central role of metabolism in regulating skeletal muscle types and functions, comprehensive metabolomics investigations focusing on the metabolic differences between these muscle types are lacking. To reveal the differences in metabolic characteristics between the SM and FM of teleost, we conducted an untargeted metabolomics analysis using Pseudocaranx dentex as a representative model and identified 411 differential metabolites (DFMs), of which 345 exhibited higher contents in SM and 66 in FM. KEGG enrichment analysis showed that these DFMs were enriched in the metabolic processes of lipids, amino acids, carbohydrates, purines, and vitamins, suggesting that there were significant differences between the SM and FM in multiple metabolic pathways, especially in the metabolism of energy substances. Furthermore, an integrative analysis of metabolite contents, enzymatic activity assays, and gene expression levels involved in ATP-PCr phosphate, anaerobic glycolysis, and aerobic oxidative energy systems was performed to explore the potential regulatory mechanisms of energy metabolism differences. The results unveiled a set of differential metabolites, enzymes, and genes between the SM and FM, providing compelling molecular evidence of the FM achieving a higher anaerobic energy supply capacity through the ATP-PCr phosphate and glycolysis energy systems, while the SM obtains greater energy supply capacity via aerobic oxidation. These findings significantly advance our understanding of the metabolic profiles and related regulatory mechanisms of skeletal muscles, thereby expanding the knowledge of metabolic physiology and ecological adaptation in teleost fish.

更新日期：2024-06-01

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