To investigate the ideal time in culture to optimize embryo cell-free deoxyribonucleic acid (cfDNA) analysis in frozen-thawed blastocysts undergoing noninvasive preimplantation genetic testing for aneuploidy (PGT-A). Cell-free DNA is released into the spent blastocyst media (spent media) by the embryo. However, the optimal timing to determine maximal cfDNA in the case of frozen-thawed blastocysts undergoing noninvasive PGT-A remains to be elucidated.

中文翻译：

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用于优化冷冻解冻囊胚胚胎无细胞 DNA 分析的培养时间，进行非整倍性非侵入性植入前基因检测


目的 研究优化冻融囊胚中胚胎无细胞脱氧核糖核酸 (cfDNA) 分析的理想培养时间，进行无创植入前非整倍体基因检测 (PGT-A)。胚胎将游离DNA释放到用过的囊胚培养基（用过的培养基）中。然而，对于接受非侵入性 PGT-A 的冻融囊胚来说，确定最大 cfDNA 的最佳时机仍有待阐明。