<br>双功能 Tb(III) 修饰 Ce-MOF 纳米探针，用于 α-葡萄糖苷酶活性的比色和荧光传感,Talanta

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双功能 Tb(III) 修饰 Ce-MOF 纳米探针，用于 α-葡萄糖苷酶活性的比色和荧光传感
Talanta ( IF 5.6 ) Pub Date : 2024-05-22 , DOI: 10.1016/j.talanta.2024.126304
Yi Xiao ₁ , Pengcheng Huang ₁ , Fang-Ying Wu ₁

Affiliation

α-葡萄糖苷酶直接参与淀粉和糖原的代谢并引起血糖水平升高，是预防和治疗II型糖尿病的主要靶酶。在前期工作的基础上，我们采用合成后修饰的方法将Tb封装到具有混合价态的Ce-MOF纳米酶中。 Tb@Ce-MOF表现出诱导发光特性和出色的类氧化酶活性，可以将无色的3,3',5,5'-四甲基联苯胺（TMB）氧化成蓝色的ox-TMB。 α-葡萄糖苷酶可以水解底物-抗坏血酸-2--α--吡喃葡萄糖基（AAG）生成抗坏血酸（AA），从而增加Tb@Ce-MOF中Ce/Ce氧化还原价态，从而导致抑制TMB 的异色反应和 ox-TMB 在 652 nm 处的吸收减少。由于Tb@Ce-MOF中Ce/Ce模式的增加，从Ce到Tb的能量转移（EnT）过程将增强，这将导致Tb@Ce-MOF在550 nm处的荧光信号增强。但抑制剂阿卡波糖的加入会抑制上述过程。我们构建了比色法和荧光法双模式检测α-葡萄糖苷酶及其抑制剂的平台。 α-葡萄糖苷酶的线性范围分别为0.01–0.5 U/mL（比色模式）和0.8–1.5 U/mL（荧光模式），检测限低至0.0018 U/mL。此外，我们的方法还成功应用于血清样品中α-葡萄糖苷酶的分析。

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Bifunctional Tb(III)-modified Ce-MOF nanoprobe for colorimetric and fluorescence sensing of α-glucosidase activity

α-Glucosidase, which directly involves in the metabolism of starch and glycogen and causes an increase in blood sugar level, is the major target enzyme for the precaution and therapy of type II diabetes. Based on the previous work, we adopted a post-synthetic modification method to encapsulate Tb into Ce-MOF nanozyme which owned mixed valence states. Tb@Ce-MOF displayed induced luminescence characteristic and exceptional oxidase-like activity that could oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB. α-Glucosidase can hydrolyze the substrate -ascorbic acid-2--α--glucopyranosyl (AAG) to generate ascorbic acid (AA), which could increase the Ce/Ce redox valence mode in Tb@Ce-MOF, leading to the inhibition of the allochroic reaction of TMB and the decreased absorption of ox-TMB at 652 nm. The energy transfer (EnT) process from Ce to Tb will enhance due to the increased Ce/Ce mode in Tb@Ce-MOF, which will result in an enhanced fluorescence signal of Tb@Ce-MOF at 550 nm. But the addition of inhibitor acarbose will inhibit the above process. We have constructed a dual-mode detection platform of α-glucosidase and its inhibitor via colorimetric and fluorometric method. The linear range of α-glucosidase were 0.01–0.5 U/mL (colorimetric mode) and 0.8–1.5 U/mL (fluorometric mode), respectively, with a detection limit as low as 0.0018 U/mL. Furthermore, our approach was also successfully employed to the analysis of α-glucosidase in serum samples.

更新日期：2024-05-22

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