Soybean [Glycine max (L.) Merr] seed is a valuable source of raw materials of protein, oil, and metabolites for food, feed, and pharmaceutical industries. Using global metabolic profiling, we identified 718 non‐redundant metabolites of a fast neutron‐mediated soybean known to have 24 genes deleted and 15% more seed protein compared to wild‐type seed. Among the 554 identified metabolites in leaves and 629 identified in seed, 465 were found in both leaves and seed. Mapping of the deleted genes and identified metabolites indicated a complex perturbation of global metabolism involving glutamine‐arginine, aspartate, aromatic amino acid, glycolytic, and tricarboxylic acid cycle (TCA) pathways. The mutant seed showed a higher content of sulfur‐containing metabolites such as S‐methylmethionine, methionine, and cysteine compared to wild‐type seed. The gene deletions may alter carbon flow from glycolytic and TCA pathways, resulting in higher protein content and increased abundance of sulfur‐containing metabolites in the mutant seeds. The results provide valuable information for altering metabolic pathways to increase value‐added seed traits in soybeans.

中文翻译：

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快中子大豆突变体的非靶向代谢物分析显示含硫代谢物增加


大豆 [Glycine max (L.) Merr] 种子是食品、饲料和制药行业蛋白质、油和代谢物原材料的宝贵来源。通过全局代谢分析，我们鉴定了快中子介导大豆的 718 种非冗余代谢物，已知该大豆删除了 24 个基因，并且与野生型种子相比种子蛋白多了 15%。在叶子中鉴定出的 554 种代谢物和种子中鉴定出的 629 种代谢物中，其中在叶子和种子中均发现了 465 种。对删除的基因和已鉴定的代谢物进行图谱分析表明，涉及谷氨酰胺-精氨酸、天冬氨酸、芳香族氨基酸、糖酵解和三羧酸循环（TCA）途径的整体代谢存在复杂的扰动。与野生型种子相比，突变种子显示出更高含量的含硫代谢物，如S-甲基蛋氨酸、蛋氨酸和半胱氨酸。基因缺失可能会改变糖酵解和 TCA 途径的碳流，从而导致突变种子中蛋白质含量更高，含硫代谢物丰度增加。研究结果为改变代谢途径以增加大豆种子的附加值性状提供了有价值的信息。