ObjectivesThe glow discharge plasma (GDP) procedure has proven efficacy in grafting allylamine onto zirconia dental implant surfaces to enhance osseointegration. This study explored the enhancement of zirconia dental implant properties using GDP at different energy settings (25, 50, 75, 100, and 200 W) both in vitro and in vivo.Materials and MethodsIn vitro analyses included scanning electron microscopy, wettability assessment, energy‐dispersive X‐ray spectroscopy, and more. In vivo experiments involved implanting zirconia dental implants into rabbit femurs and later evaluation through impact stability test, micro‐CT, and histomorphometric measurements.ResultsThe results demonstrated that 25 and 50 W GDP allylamine grafting positively impacted MG‐63 cell proliferation and increased alkaline phosphatase activity. Gene expression analysis revealed upregulation of OCN, OPG, and COL‐I. Both 25 and 50 W GDP allylamine grafting significantly improved zirconia's surface properties (p < .05, p < .01, p < .001). However, only 25 W allylamine grafting with optimal energy settings promoted in vivo osseointegration and new bone formation while preventing bone level loss around the dental implant (p < .05, p < .01, p < .001).ConclusionsThis study presents a promising method for enhancing Zr dental implant surface's bioactivity.

中文翻译：

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氧化锆牙种植体表面的烯丙胺涂层可促进体外成骨分化并加速体内骨整合


目的 辉光放电等离子体 (GDP) 程序已被证明能够将烯丙胺移植到氧化锆牙科种植体表面以增强骨整合。本研究探讨了在体外和体内不同能量设置（25、50、75、100 和 200 W）下使用 GDP 来增强氧化锆牙种植体性能。材料和方法体外分析包括扫描电子显微镜、润湿性评估、能量‐色散 X 射线光谱学等。体内实验包括将氧化锆牙种植体植入兔股骨中，然后通过冲击稳定性测试、显微 CT 和组织形态测量进行评估。结果结果表明，25 和 50 W GDP 烯丙胺移植对 MG-63 细胞增殖产生积极影响，并增加碱性磷酸酶活性。基因表达分析显示 OCN、OPG 和 COL-I 上调。 25 和 50 W GDP 烯丙胺接枝均显着改善了氧化锆的表面性能 (p < .05、p < .01、p < .001)。然而，只有具有最佳能量设置的 25 W 烯丙胺移植才能促进体内骨整合和新骨形成，同时防止牙种植体周围骨水平丢失 (p < .05、p < .01、p < .001)。结论本研究提出了一种增强锆牙种植体表面生物活性的有前途的方法。