The integrity of the intestinal barrier is crucial for regulating the passage of pathogens and toxins, while facilitating nutrient absorption. The everted gut sac technique, an ex-vivo technique, can be used to study interventions on barrier function. This cost-effective approach utilizes relatively large gut segments to study specific intestinal regions. Typically, intact (non-stripped) intestinal segments are used, but their use may underestimate permeability due to the medial positioning of blood vessels relative to the seromuscular layer and serosa. However, removing these layers risks physical damage, resulting in an overestimation of intestinal permeability. Therefore, we investigated the impact of stripping jejunal segments on permeability to fluorescein isothiocynate-dextran (FITC, 4 kDa) and tetramethylrhodamine isothiocynate-dextran (TRITC, 40 kDa), and on the absorption of glucose, lysine, and methionine in jejunal segments from 80 piglets at 8 days post-weaning. Piglets were subjected to either high or low sanitary housing conditions and diets provoking intestinal protein fermentation or not, expected to influence intestinal permeability. Stripping of the seromuscular layer and serosa increased the passage of 4 kDa FITC-dextran (stripped vs. non-stripped; 1.1 vs. 0.9 pmol/cm2/min, P<0.001), glucose (40.0 vs. 19.1 pmol/cm2/min, P<0.001), lysine (2.5 vs. 2.0 nmol/cm2/min, P<0.001), and methionine (4.1 vs. 2.7 pmol/cm2/min, P<0.001). As permeability increased, the differences in methionine passage between stripped and non-stripped intestinal segments also increased (slope = 1.30, P=0.009). The coefficients of variation were comparable between stripped and non-stripped intestines (over all treatments, stripped vs. non-stripped 38 vs. 40%). Stripping, by isolating mucosal processes without introducing additional variation, is thus recommended for studies on intestinal permeability or absorption.

中文翻译：

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简短的交流：评估断奶仔猪外翻囊段肠道通透性测量的浆肌层和浆膜去除


肠道屏障的完整性对于调节病原体和毒素的通过以及促进营养吸收至关重要。外翻肠囊技术是一种离体技术，可用于研究对屏障功能的干预措施。这种经济有效的方法利用相对较大的肠道片段来研究特定的肠道区域。通常，使用完整的（未剥离的）肠段，但由于血管相对于浆膜层和浆膜位于内侧，因此使用它们可能会低估渗透性。然而，去除这些层可能会造成物理损伤，从而导致肠道渗透性的高估。因此，我们研究了剥离空肠段对异硫氰酸荧光素-葡聚糖（FITC，4 kDa）和异硫氰酸四甲基罗丹明-葡聚糖（TRITC，40 kDa）渗透性的影响，以及对空肠段中葡萄糖、赖氨酸和蛋氨酸吸收的影响。 80头断奶后8天的仔猪。仔猪接受高或低卫生条件的饲养条件以及是否引发肠道蛋白质发酵的饮食，预计会影响肠道通透性。浆膜层和浆膜的剥离增加了 4 kDa FITC-葡聚糖的通过（剥离与未剥离；1.1 vs. 0.9 pmol/cm2/min，P<0.001）、葡萄糖（40.0 vs. 19.1 pmol/cm2） /min，P<0.001)、赖氨酸（2.5 vs. 2.0 nmol/cm2/min，P<0.001）和蛋氨酸（4.1 vs. 2.7 pmol/cm2/min，P<0.001）。随着渗透性的增加，剥离和未剥离肠段之间蛋氨酸通过的差异也增加（斜率= 1.30，P= 0.009）。剥离肠和未剥离肠之间的变异系数相当（在所有处理中，剥离肠与未剥离肠分别为 38% 和 40%）。 因此，建议通过分离粘膜过程而不引入额外的变化来进行剥离，以用于肠道渗透性或吸收的研究。